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- W1970361690 abstract "High-dilution equilibrium macrocyclization is developed as a general approach to trapping proteins in a non-native state with a synthetic cross-linking agent. The approach is illustrated using the N-terminal domain of phosphoglycerate kinase and a synthetic reagent containing two maleimide groups, for selective attachment to cysteines introduced onto the protein surface through mutagenesis, and an aromatic disulfide that can be chemically or photochemically cleaved. Following functionalization of the cysteine residues, thiol−disulfide exchange chemistry under strongly unfolding conditions was used to achieve intramolecular cyclization and a high yield of the cross-linked protein. 1H NMR, CD, and fluorescence spectroscopies indicate that the conformation of the cross-linked protein is non-native. Chemical cleavage of the aromatic disulfide cross-link by a reducing agent results in the acquisition of a nativelike conformation for the reduced protein. Thus, the cross-link acts as a reversible switch of protein folding." @default.
- W1970361690 created "2016-06-24" @default.
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- W1970361690 date "2008-11-26" @default.
- W1970361690 modified "2023-09-27" @default.
- W1970361690 title "A Method for the Reversible Trapping of Proteins in Non-Native Conformations" @default.
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- W1970361690 doi "https://doi.org/10.1021/bi801362f" @default.
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