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- W1970412489 abstract "A rapid, miniature thin-layer chromatographic procedure has been developed for the separation of unconjugated tri- and dihydroxy bile acids. The new solvent system consisted of isooctane:diisopropyl ether:methanol:acetic acid:formamide (2:1:0.13:0.07:0.02,vv). Complete separation of tri- and dihydroxy bile acid fractions was easily achieved within 1 min on ITLC type SG chromatography sheets. A sensitive fluorescent visualization technique was employed. The reflected fluorescence intensity of the bile acids fluorophore spots was measured with a Farrand Mark I spectrofluorometer equipped with a thin-layer scanning attachment. The excitation and emission wavelengths were 375 and 436 nm, respectively. The above procedure has been adapted for the separation of unconjugated bile acids from serum samples. The bile acids were adsorbed onto Amberlite XAD-2, eluted with ethanol, and concentrated. Further purification was performed by a simple 3-min thin-layer chromatographic technique. This new procedure for the separation of tri- and dihydroxy bile acids is rapid, sensitive, and less complex than conventional procedures presently employed in clinical laboratories." @default.
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- W1970412489 date "1981-06-01" @default.
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- W1970412489 title "A highly effective one-minute thin-layer chromatographic separation of unconjugated tri- and dihydroxy bile acids" @default.
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- W1970412489 doi "https://doi.org/10.1016/0026-265x(81)90099-0" @default.
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