Matches in SemOpenAlex for { <https://semopenalex.org/work/W1970427785> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W1970427785 endingPage "2319" @default.
- W1970427785 startingPage "2312" @default.
- W1970427785 abstract "A general scheme for the purification of baculovirus-expressed cytochrome P450s (P450s) from the crude insect cell pastes has been designed which renders the P450s suitable for analysis by high-performance liquid chromatography (HPLC) electrospray ionization mass spectrometry (ESI-MS). An HPLC/ESI-MS procedure has been developed to analyze small amounts of intact purified P450 (P450s cam-HT, 1A1, 1A2, 2A6, 2B1, 2C9, 2C9 C175R, 3A4, 3A4-HT) and rat NADPH cytochrome P450 reductase (P450 reductase). The experimentally determined and predicted (based on the amino acid sequences) molecular masses (MMs) of the various proteins had identical rank orders. For each individual protein, the difference between the experimentally determined (+/-SD, based on experiments performed on at least 3 different days) and predicted MMs ranged from 0.002 to 0.035%. Each experimentally determined MM had a standard deviation of less than 0.09% (based on the charge state distribution). Application of this HPLC/ESI-MS technique made the detection of the covalent modification to P450 2C9 following mechanism-based inactivation by tienilic acid possible. In the absence of glutathione, three P450 2C9 species were detected that produced ESI mass spectra corresponding to native P450 2C9 and both a monoadduct and a diadduct of tienilic acid to P450 2C9. In the presence of glutathione, only native P450 2C9 and the monoadduct were detected. Based on the observed mass shifts for the P450 2C9/tienilic acid adducts, a mechanism for the inactivation of P450 2C9 by tienilic acid is proposed." @default.
- W1970427785 created "2016-06-24" @default.
- W1970427785 creator A5006891316 @default.
- W1970427785 creator A5018443721 @default.
- W1970427785 creator A5025093438 @default.
- W1970427785 creator A5038247773 @default.
- W1970427785 creator A5045987787 @default.
- W1970427785 creator A5056861525 @default.
- W1970427785 creator A5062454264 @default.
- W1970427785 creator A5066900523 @default.
- W1970427785 creator A5075729159 @default.
- W1970427785 creator A5088659697 @default.
- W1970427785 date "1999-02-01" @default.
- W1970427785 modified "2023-09-23" @default.
- W1970427785 title "Electrospray Ionization Mass Spectrometric Analysis of Intact Cytochrome P450: Identification of Tienilic Acid Adducts to P450 2C9" @default.
- W1970427785 cites W1567686906 @default.
- W1970427785 cites W1871379037 @default.
- W1970427785 cites W1951469402 @default.
- W1970427785 cites W2050005113 @default.
- W1970427785 doi "https://doi.org/10.1021/bi9823030" @default.
- W1970427785 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10029524" @default.
- W1970427785 hasPublicationYear "1999" @default.
- W1970427785 type Work @default.
- W1970427785 sameAs 1970427785 @default.
- W1970427785 citedByCount "88" @default.
- W1970427785 countsByYear W19704277852012 @default.
- W1970427785 countsByYear W19704277852014 @default.
- W1970427785 countsByYear W19704277852015 @default.
- W1970427785 countsByYear W19704277852016 @default.
- W1970427785 countsByYear W19704277852017 @default.
- W1970427785 countsByYear W19704277852020 @default.
- W1970427785 countsByYear W19704277852021 @default.
- W1970427785 countsByYear W19704277852022 @default.
- W1970427785 crossrefType "journal-article" @default.
- W1970427785 hasAuthorship W1970427785A5006891316 @default.
- W1970427785 hasAuthorship W1970427785A5018443721 @default.
- W1970427785 hasAuthorship W1970427785A5025093438 @default.
- W1970427785 hasAuthorship W1970427785A5038247773 @default.
- W1970427785 hasAuthorship W1970427785A5045987787 @default.
- W1970427785 hasAuthorship W1970427785A5056861525 @default.
- W1970427785 hasAuthorship W1970427785A5062454264 @default.
- W1970427785 hasAuthorship W1970427785A5066900523 @default.
- W1970427785 hasAuthorship W1970427785A5075729159 @default.
- W1970427785 hasAuthorship W1970427785A5088659697 @default.
- W1970427785 hasConcept C108204754 @default.
- W1970427785 hasConcept C162356407 @default.
- W1970427785 hasConcept C178790620 @default.
- W1970427785 hasConcept C179998833 @default.
- W1970427785 hasConcept C181199279 @default.
- W1970427785 hasConcept C185592680 @default.
- W1970427785 hasConcept C194451485 @default.
- W1970427785 hasConcept C40684141 @default.
- W1970427785 hasConcept C43617362 @default.
- W1970427785 hasConcept C526171541 @default.
- W1970427785 hasConcept C538909803 @default.
- W1970427785 hasConcept C55493867 @default.
- W1970427785 hasConceptScore W1970427785C108204754 @default.
- W1970427785 hasConceptScore W1970427785C162356407 @default.
- W1970427785 hasConceptScore W1970427785C178790620 @default.
- W1970427785 hasConceptScore W1970427785C179998833 @default.
- W1970427785 hasConceptScore W1970427785C181199279 @default.
- W1970427785 hasConceptScore W1970427785C185592680 @default.
- W1970427785 hasConceptScore W1970427785C194451485 @default.
- W1970427785 hasConceptScore W1970427785C40684141 @default.
- W1970427785 hasConceptScore W1970427785C43617362 @default.
- W1970427785 hasConceptScore W1970427785C526171541 @default.
- W1970427785 hasConceptScore W1970427785C538909803 @default.
- W1970427785 hasConceptScore W1970427785C55493867 @default.
- W1970427785 hasIssue "8" @default.
- W1970427785 hasLocation W19704277851 @default.
- W1970427785 hasLocation W19704277852 @default.
- W1970427785 hasOpenAccess W1970427785 @default.
- W1970427785 hasPrimaryLocation W19704277851 @default.
- W1970427785 hasRelatedWork W1969034512 @default.
- W1970427785 hasRelatedWork W1980225556 @default.
- W1970427785 hasRelatedWork W2014605936 @default.
- W1970427785 hasRelatedWork W2027593566 @default.
- W1970427785 hasRelatedWork W2095026591 @default.
- W1970427785 hasRelatedWork W2107172911 @default.
- W1970427785 hasRelatedWork W2133074682 @default.
- W1970427785 hasRelatedWork W2165290697 @default.
- W1970427785 hasRelatedWork W2379012137 @default.
- W1970427785 hasRelatedWork W4318586533 @default.
- W1970427785 hasVolume "38" @default.
- W1970427785 isParatext "false" @default.
- W1970427785 isRetracted "false" @default.
- W1970427785 magId "1970427785" @default.
- W1970427785 workType "article" @default.