FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1970463795> ?p ?o ?g. }

• W1970463795 abstract "CpG islands (CGIs) in human genomic DNA are GC-rich fragments whose aberrant methylation is associated with human disease development. In the current study, methylation-sensitive mirror orientation selection (MS-MOS) was developed to efficiently isolate and enrich unmethylated CGIs from human genomic DNA. The unmethylated CGIs prepared by the MS-MOS procedure subsequently were used to construct a CGI library. Then the sequence characteristics of cloned inserts of the library were analyzed by bioinformatics tools, and the methylation status of CGI clones was analyzed by HpaII PCR. The results showed that the MS-MOS method could be used to isolate up to 0.001% of differentially existed unmethylated DNA fragments in two complex genomic DNA. In the CGI library, 34.1% of clones had insert sequences satisfying the minimal criteria for CGIs. Excluding duplicates, 22.0% of the 80,000 clones were unique CGI clones, representing 60% of all the predicted CGIs (about 29,000) in human genomic DNA, and most or all of the CGI clones were unmethylated in human normal cell DNA based on the HpaII PCR analysis results of randomly selected CGI clones. In conclusion, MS-MOS was an efficient way to isolate and enrich human genomic CGIs. The method has powerful potential application in the comprehensive identification of aberrantly methylated CGIs associated with human tumorigenesis to improve understanding of the epigenetic mechanisms involved." @default.
• W1970463795 created "2016-06-24" @default.
• W1970463795 creator A5007757773 @default.
• W1970463795 creator A5030171355 @default.
• W1970463795 creator A5037602041 @default.
• W1970463795 creator A5045858690 @default.
• W1970463795 creator A5049030739 @default.
• W1970463795 creator A5049542498 @default.
• W1970463795 creator A5050645206 @default.
• W1970463795 creator A5056401944 @default.
• W1970463795 creator A5064523767 @default.
• W1970463795 creator A5076036593 @default.
• W1970463795 creator A5082921671 @default.
• W1970463795 creator A5085662381 @default.
• W1970463795 date "2007-06-01" @default.
• W1970463795 modified "2023-09-22" @default.
• W1970463795 title "Isolation and enrichment of human genomic CpG islands by methylation-sensitive mirror orientation selection" @default.
• W1970463795 cites W1556996865 @default.
• W1970463795 cites W1571792157 @default.
• W1970463795 cites W1752198036 @default.
• W1970463795 cites W1755052449 @default.
• W1970463795 cites W1787537093 @default.
• W1970463795 cites W1903840298 @default.
• W1970463795 cites W1965647561 @default.
• W1970463795 cites W1969304546 @default.
• W1970463795 cites W1979613482 @default.
• W1970463795 cites W1983238182 @default.
• W1970463795 cites W1987523545 @default.
• W1970463795 cites W1987615161 @default.
• W1970463795 cites W1992168269 @default.
• W1970463795 cites W1998517881 @default.
• W1970463795 cites W1999119486 @default.
• W1970463795 cites W2002292866 @default.
• W1970463795 cites W2009121506 @default.
• W1970463795 cites W2012617920 @default.
• W1970463795 cites W2014955899 @default.
• W1970463795 cites W2029868343 @default.
• W1970463795 cites W2034641677 @default.
• W1970463795 cites W2037920286 @default.
• W1970463795 cites W2051166209 @default.
• W1970463795 cites W2061839857 @default.
• W1970463795 cites W2079043502 @default.
• W1970463795 cites W2084637809 @default.
• W1970463795 cites W2084748297 @default.
• W1970463795 cites W2087079130 @default.
• W1970463795 cites W2090701958 @default.
• W1970463795 cites W2091414779 @default.
• W1970463795 cites W2104429441 @default.
• W1970463795 cites W2110319986 @default.
• W1970463795 cites W2111183991 @default.
• W1970463795 cites W2127230663 @default.
• W1970463795 cites W2130809733 @default.
• W1970463795 cites W2133142557 @default.
• W1970463795 cites W2137505881 @default.
• W1970463795 cites W2145457002 @default.
• W1970463795 cites W2150651400 @default.
• W1970463795 cites W2152869198 @default.
• W1970463795 cites W2154929782 @default.
• W1970463795 cites W2168909179 @default.
• W1970463795 cites W2172215791 @default.
• W1970463795 cites W2278272055 @default.
• W1970463795 cites W2407068850 @default.
• W1970463795 cites W2804007336 @default.
• W1970463795 doi "https://doi.org/10.1016/j.ab.2007.03.035" @default.
• W1970463795 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17481566" @default.
• W1970463795 hasPublicationYear "2007" @default.
• W1970463795 type Work @default.
• W1970463795 sameAs 1970463795 @default.
• W1970463795 citedByCount "1" @default.
• W1970463795 crossrefType "journal-article" @default.
• W1970463795 hasAuthorship W1970463795A5007757773 @default.
• W1970463795 hasAuthorship W1970463795A5030171355 @default.
• W1970463795 hasAuthorship W1970463795A5037602041 @default.
• W1970463795 hasAuthorship W1970463795A5045858690 @default.
• W1970463795 hasAuthorship W1970463795A5049030739 @default.
• W1970463795 hasAuthorship W1970463795A5049542498 @default.
• W1970463795 hasAuthorship W1970463795A5050645206 @default.
• W1970463795 hasAuthorship W1970463795A5056401944 @default.
• W1970463795 hasAuthorship W1970463795A5064523767 @default.
• W1970463795 hasAuthorship W1970463795A5076036593 @default.
• W1970463795 hasAuthorship W1970463795A5082921671 @default.
• W1970463795 hasAuthorship W1970463795A5085662381 @default.
• W1970463795 hasConcept C104317684 @default.
• W1970463795 hasConcept C127413603 @default.
• W1970463795 hasConcept C13575015 @default.
• W1970463795 hasConcept C140173407 @default.
• W1970463795 hasConcept C141231307 @default.
• W1970463795 hasConcept C150194340 @default.
• W1970463795 hasConcept C153911025 @default.
• W1970463795 hasConcept C17757408 @default.
• W1970463795 hasConcept C189206191 @default.
• W1970463795 hasConcept C190727270 @default.
• W1970463795 hasConcept C197077220 @default.
• W1970463795 hasConcept C2777162945 @default.
• W1970463795 hasConcept C2780098074 @default.
• W1970463795 hasConcept C3017666073 @default.
• W1970463795 hasConcept C33288867 @default.
• W1970463795 hasConcept C41091548 @default.
• W1970463795 hasConcept C54355233 @default.
• W1970463795 hasConcept C552990157 @default.

• next