Matches in SemOpenAlex for { <https://semopenalex.org/work/W1970467956> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W1970467956 endingPage "1961" @default.
- W1970467956 startingPage "1961" @default.
- W1970467956 abstract "It has been shown that myocardial tissue function may be better preserved if antioxidants are incorporated into the reoxygenation medium at the end of the ischemic period following isolation of the organ. Although isolated pancreatic islets are prone to ischemic-reperfusion injury, it is not clear if antioxidants have a role in the preservation of their function. The purpose of the present study, therefore, was to examine the effect of the addition of glutathione (GSH) to a physiologic incubation medium on pancreatic islet response to glucose stimulation. Islets isolated by microdissection were preperifused at the rate of 1 ml/min for 1 hr at 37°C, with Krebs-Ringer bicarbonate (KRB) buffer containing 1% albumin, 5.5 mM (basal) glucose without (control) or with 10 mM glutamine or 10 mM GSH and maintained at pH 7.4 by continuous gassing with 95/5% O2/CO2. After preperifusion, basal effluent samples were taken on ice for 20 min. The perifusion was then continued for 20 min with the KRB containing 27.7 mM glucose alone, followed by another 20 min of basal glucose washout. Solutions were changed using a stopcock and all effluent perifusate samples obtained were stored frozen at -20°C until radioimmunoassay for insulin. Total insulin output in the control group increased from a basal 11.45 ± 3.18 to 29.23 ± 7.08 ng/6 islets/20 min (P < 0.001, n = 5) when the glucose concentration was raised to 27.7 mM. During a 20-min washout, insulin secretion was still significantly raised and did not return to the prestimulation basal rate. In the glutamine-treated islets, insulin output increased from 7.23 ± 0.94 to 16.83 ± 2.25 ng/6 islets/20 min (P < 0.001, n = 5) with 27.7 mM glucose stimulation and the significantly raised washout basal rate of secretion did not return to the prestimulation level. GSH treatment not only caused an enhanced 27.7 mM glucose stimulation (8.46 ± 1.99 to 38.72 ± 11.51 ng/6 islets/20 min, P < 0.001, n = 6) of insulin output but also completely restored the basal rate of insulin secretion to the prestimulation level within the 20-min washout perifusion. In conclusion, these data show that incubation of isolated islets with GSH enhanced their secretory response to glucose stimulation and preserved their functional integrity." @default.
- W1970467956 created "2016-06-24" @default.
- W1970467956 creator A5016758593 @default.
- W1970467956 creator A5017318032 @default.
- W1970467956 creator A5031163174 @default.
- W1970467956 creator A5060727331 @default.
- W1970467956 creator A5074156404 @default.
- W1970467956 creator A5077962224 @default.
- W1970467956 date "1997-06-01" @default.
- W1970467956 modified "2023-09-25" @default.
- W1970467956 title "Efficacy of a new collagenase for canine and murine islet isolation" @default.
- W1970467956 doi "https://doi.org/10.1016/s0041-1345(97)00181-4" @default.
- W1970467956 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9193476" @default.
- W1970467956 hasPublicationYear "1997" @default.
- W1970467956 type Work @default.
- W1970467956 sameAs 1970467956 @default.
- W1970467956 citedByCount "2" @default.
- W1970467956 crossrefType "journal-article" @default.
- W1970467956 hasAuthorship W1970467956A5016758593 @default.
- W1970467956 hasAuthorship W1970467956A5017318032 @default.
- W1970467956 hasAuthorship W1970467956A5031163174 @default.
- W1970467956 hasAuthorship W1970467956A5060727331 @default.
- W1970467956 hasAuthorship W1970467956A5074156404 @default.
- W1970467956 hasAuthorship W1970467956A5077962224 @default.
- W1970467956 hasConcept C126322002 @default.
- W1970467956 hasConcept C134018914 @default.
- W1970467956 hasConcept C165220095 @default.
- W1970467956 hasConcept C176685330 @default.
- W1970467956 hasConcept C181199279 @default.
- W1970467956 hasConcept C185592680 @default.
- W1970467956 hasConcept C2778024521 @default.
- W1970467956 hasConcept C2778394429 @default.
- W1970467956 hasConcept C2779306644 @default.
- W1970467956 hasConcept C2779349466 @default.
- W1970467956 hasConcept C2781112554 @default.
- W1970467956 hasConcept C515207424 @default.
- W1970467956 hasConcept C55493867 @default.
- W1970467956 hasConcept C71839028 @default.
- W1970467956 hasConcept C71924100 @default.
- W1970467956 hasConcept C86803240 @default.
- W1970467956 hasConceptScore W1970467956C126322002 @default.
- W1970467956 hasConceptScore W1970467956C134018914 @default.
- W1970467956 hasConceptScore W1970467956C165220095 @default.
- W1970467956 hasConceptScore W1970467956C176685330 @default.
- W1970467956 hasConceptScore W1970467956C181199279 @default.
- W1970467956 hasConceptScore W1970467956C185592680 @default.
- W1970467956 hasConceptScore W1970467956C2778024521 @default.
- W1970467956 hasConceptScore W1970467956C2778394429 @default.
- W1970467956 hasConceptScore W1970467956C2779306644 @default.
- W1970467956 hasConceptScore W1970467956C2779349466 @default.
- W1970467956 hasConceptScore W1970467956C2781112554 @default.
- W1970467956 hasConceptScore W1970467956C515207424 @default.
- W1970467956 hasConceptScore W1970467956C55493867 @default.
- W1970467956 hasConceptScore W1970467956C71839028 @default.
- W1970467956 hasConceptScore W1970467956C71924100 @default.
- W1970467956 hasConceptScore W1970467956C86803240 @default.
- W1970467956 hasIssue "4" @default.
- W1970467956 hasLocation W19704679561 @default.
- W1970467956 hasLocation W19704679562 @default.
- W1970467956 hasOpenAccess W1970467956 @default.
- W1970467956 hasPrimaryLocation W19704679561 @default.
- W1970467956 hasRelatedWork W160797072 @default.
- W1970467956 hasRelatedWork W1973049774 @default.
- W1970467956 hasRelatedWork W1994826134 @default.
- W1970467956 hasRelatedWork W2000995372 @default.
- W1970467956 hasRelatedWork W2012430287 @default.
- W1970467956 hasRelatedWork W2026121087 @default.
- W1970467956 hasRelatedWork W2064538767 @default.
- W1970467956 hasRelatedWork W2069827981 @default.
- W1970467956 hasRelatedWork W2080189545 @default.
- W1970467956 hasRelatedWork W2130116832 @default.
- W1970467956 hasVolume "29" @default.
- W1970467956 isParatext "false" @default.
- W1970467956 isRetracted "false" @default.
- W1970467956 magId "1970467956" @default.
- W1970467956 workType "article" @default.