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• W1970490057 abstract "Sodium/proton exchangers (NHE) are transmembrane proteins that facilitate the exchange of a Na(+) ion for a H(+) ion across cellular membranes. The NHE are present in the gills of fishes and are believed to function in acid-base regulation by driving the extrusion of protons across the branchial epithelium in exchange for Na(+) in the water. In this study, we have used reverse transcriptase-polymerase chain reaction (RT-PCR) to detect the presence of a branchial NHE in the gills of the Atlantic hagfish, Myxine glutinosa. The subsequent partial cDNA sequence shares homology with other vertebrate and invertebrate NHE isoforms. In addition, using semi-quantitative, multiplex RT-PCR we demonstrate that mRNA expression of hagfish gill NHE is upregulated following an induced metabolic acidosis. Expression was increased to 4.4 times basal levels at 2-h post-infusion and had decreased to 1.6 times basal by 6 h. Expression had returned to basal levels by 24-h post-infusion. The inference from this study is that a gill NHE which is potentially important in acid-base regulation has been present in the vertebrate lineage since before the divergence of the hagfishes from the main vertebrate line." @default.
• W1970490057 created "2016-06-24" @default.
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• W1970490057 date "2001-08-01" @default.
• W1970490057 modified "2023-10-16" @default.
• W1970490057 title "Expression of Na+/H+ exchanger mRNA in the gills of the Atlantic hagfish (Myxine glutinosa) in response to metabolic acidosis" @default.
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• W1970490057 doi "https://doi.org/10.1016/s1095-6433(01)00367-1" @default.
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