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• W1970635203 abstract "To determine whether the process of sperm nuclear destabilization would begin before sperm-oocyte fusion in humans.Changes in the distribution of human protamine 1 were investigated in human spermatozoa from the ejaculate, in spermatozoa selected by swim-up or Percoll techniques, and in spermatozoa bound to zona pellucida (ZP) from oocytes that failed to fertilize in an IVF program.Center for Infertility and Assisted Reproductive Technology, and university departments.Fifteen couples undergoing an IVF program.Women underwent a similar superovulation induction protocol that consisted of GnRH agonist associated with hMG.Comparative immunoelectron microscopic study of sperm nucleus labeling with an anti-human protamine 1 specific protamine monoclonal antibody.After selection by swim-up or by Percoll, spermatozoa show a significantly lower nuclear labeling than in the ejaculate. After binding to the ZP, labeling increases, more in spermatozoa selected by swim-up than by Percoll, but, after Percoll selection, labeling in zona-bound spermatozoa is lower than in the ejaculate.In humans sperm binding to the ZP induces differences in the accessibility of the anti-human protamine 1 antibody, which are consistent with structural rearrangements of the DNA-nucleoproteins complex. These modifications must be a prelude to sperm decondensation, protamines replacement by histones, and subsequent reactivation of the sperm genome in the oocyte." @default.
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• W1970635203 date "1997-01-01" @default.
• W1970635203 modified "2023-09-30" @default.
• W1970635203 title "Changes in protamine 1 distribution in human sperm nucleus during in vitro sperm-oocyte interaction: An immunoelectron microscopic study" @default.
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• W1970635203 doi "https://doi.org/10.1016/s0015-0282(97)81868-7" @default.
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