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- W1970663932 abstract "Blood lymphocytes from guinea pigs and human donors were incubated with varying amounts of soluble Con A or, in the human system, with Sepharose-bound Con A for 60 min and 24 h at 310 K. The cellfree lymphocyte supernatants were incubated with guinea pig peritoneal macrophages which were measured in a cell electrophoresis apparatus Parmoquant II to detect lymphokine activity. Differences were found in the slowing capacity of short-term (60 min) and long term (24 h) incubations. In the latter mobility reduction was higher at comparable Con A concentrations. Greatest macrophage slowing was found at Con A concentrations of 5--10 micrograms Con A/10(6) lymphocytes in the guinea pig system and of 40--50 micrograms Con A/10(6) lymphocytes in the human system. But there was no great difference in the heights of the macrophage slowing at the optimal Con A concentration indicating no species restriction for human lymphokine(s) in our experiments. Stimulation of lymphocytes seems to be possible with Sepharose-bound Con A during a long-term incubation as tested in the human system." @default.
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- W1970663932 date "1982-01-01" @default.
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- W1970663932 title "Studies on lymphokine activity in Coneanavalin A-stimulated lymphocyte supernatants using the macrophage-electrophoretic-mobility-test (MEM-Test)" @default.
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- W1970663932 doi "https://doi.org/10.1016/s0232-1513(82)80064-9" @default.
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