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• W1970665677 abstract "Abstract Regulation of programmed cell death by Bcl‐x L is dependent on both its solution and integral membrane conformations. A conformational change from solution to membrane is also important in this regulation. This conformational change shows a pH‐dependence similar to the translocation domain of diphtheria toxin, where an acid‐induced molten globule conformation in the absence of lipid vesicles mediates the change from solution to membrane conformations. By contrast, Bcl‐x L ΔTM in the absence of lipid vesicles exhibits no gross conformational changes upon acidification as observed by near‐ and far‐UV circular dichroism spectropolarimetry. Additionally, no significant local conformational changes upon acidification were observed by heteronuclear NMR spectroscopy of Bcl‐x L ΔTM. Under conditions that favor the solution conformation (pH 7.4), the free energy of folding for Bcl‐x L ΔTM (Δ G °) was determined to be 15.8 kcal·mol −1 . Surprisingly, under conditions that favor a membrane conformation (pH 4.9), Δ G ° was 14.6 kcal·mol −1 . These results differ from those obtained with many other membrane‐insertable proteins where acid‐induced destabilization is important. Therefore, other contributions must be necessary to destabilize the solution conformation Bcl‐x L and favor the membrane conformation at pH 4.9. Such contributions might include the presence of a negatively charged membrane or an electrostatic potential across the membrane. Thus, for proteins that adopt both solution and membrane conformations, an obligatory molten globule intermediate may not be necessary. The absence of a molten globule intermediate might have evolved to protect Bcl‐x L from intracellular proteases as it undergoes this conformational change essential for its activity." @default.
• W1970665677 created "2016-06-24" @default.
• W1970665677 creator A5016449383 @default.
• W1970665677 creator A5086151624 @default.
• W1970665677 date "2006-02-01" @default.
• W1970665677 modified "2023-09-30" @default.
• W1970665677 title "Acid destabilization of the solution conformation of Bcl-X_Ldoes not drive its pH-dependent insertion into membranes" @default.
• W1970665677 cites W1566777539 @default.
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• W1970665677 cites W1824377481 @default.
• W1970665677 cites W1851315005 @default.
• W1970665677 cites W1963773907 @default.
• W1970665677 cites W1964617786 @default.
• W1970665677 cites W1965217295 @default.
• W1970665677 cites W1965343343 @default.
• W1970665677 cites W1966225694 @default.
• W1970665677 cites W1968501522 @default.
• W1970665677 cites W1971413967 @default.
• W1970665677 cites W1972362388 @default.
• W1970665677 cites W1972547501 @default.
• W1970665677 cites W1981063713 @default.
• W1970665677 cites W1983622504 @default.
• W1970665677 cites W1992966449 @default.
• W1970665677 cites W1994611236 @default.
• W1970665677 cites W1996341064 @default.
• W1970665677 cites W1998595310 @default.
• W1970665677 cites W2003472250 @default.
• W1970665677 cites W2008045895 @default.
• W1970665677 cites W2012974735 @default.
• W1970665677 cites W2013175530 @default.
• W1970665677 cites W2015798451 @default.
• W1970665677 cites W2019572526 @default.
• W1970665677 cites W2020031720 @default.
• W1970665677 cites W2020063004 @default.
• W1970665677 cites W2020387146 @default.
• W1970665677 cites W2025421839 @default.
• W1970665677 cites W2027339215 @default.
• W1970665677 cites W2028231353 @default.
• W1970665677 cites W2028587292 @default.
• W1970665677 cites W2031315018 @default.
• W1970665677 cites W2033873142 @default.
• W1970665677 cites W2034637992 @default.
• W1970665677 cites W2034999801 @default.
• W1970665677 cites W2038263678 @default.
• W1970665677 cites W2039552447 @default.
• W1970665677 cites W2039617999 @default.
• W1970665677 cites W2043556481 @default.
• W1970665677 cites W2044328480 @default.
• W1970665677 cites W2046712651 @default.
• W1970665677 cites W2051534401 @default.
• W1970665677 cites W2066576270 @default.
• W1970665677 cites W2073854164 @default.
• W1970665677 cites W2081194139 @default.
• W1970665677 cites W2082176639 @default.
• W1970665677 cites W2083012496 @default.
• W1970665677 cites W2083645821 @default.
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• W1970665677 doi "https://doi.org/10.1110/ps.051807706" @default.
• W1970665677 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1752203" @default.
• W1970665677 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16385002" @default.
• W1970665677 hasPublicationYear "2006" @default.
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