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• W1970769416 endingPage "764" @default.
• W1970769416 startingPage "751" @default.
• W1970769416 abstract "Inhibins and activins are dimeric peptide hormones that regulate the circulating levels of follicle-stimulating hormone (FSH). In turn, FSH stimulates inhibin gene expression in the ovarian follicle; studies to date suggest that this effect is mediated by cAMP and that a cAMP-responsive element, identified in the 5′-flanking region of the α-inhibin gene, at least partially effects this response. To explore further the transcriptional regulation of the inhibin/activin genes, we have isolated and sequenced the 5′-flanking regions of the bovine α-, βA- and βB-inhibin/activin subunit genes and have analysed these regions by primer-extension analysis and DNase I footprinting with the transcription factor AP-2. Analyses indicated that all three gene promoter regions have a number of AP-2-binding sites that are resistant to competition by poly(dI-dC), suggesting that cAMP may control the inhibin/activin ratio by operating through alternative signal-transduction pathways or that inhibin/activin gene expression may be controlled by signals operating through the protein kinase C pathway. A comparison of the DNA sequences protected by AP-2 against DNase I digestion revealed a consensus AP-2-binding site of 5′-GSCCCDSS-3′, where S represents a base pairing involving three (C or G) hydrogen bonds and D represents any base other than C. The nucleotide sequences of the bovine β-subunit structural genes also are reported." @default.
• W1970769416 created "2016-06-24" @default.
• W1970769416 creator A5036440403 @default.
• W1970769416 creator A5061101357 @default.
• W1970769416 creator A5080530953 @default.
• W1970769416 date "1994-12-01" @default.
• W1970769416 modified "2023-10-11" @default.
• W1970769416 title "Genomic Cloning and Sequence Analyses of the Bovine alpha-, betaA- and betaB-Inhibin/activin Genes. Identification of Transcription Factor AP-2-Binding Sites in the 5'-Flanking Regions by DNase I Footprinting" @default.
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• W1970769416 doi "https://doi.org/10.1111/j.1432-1033.1994.00751.x" @default.
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