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- W1970781932 abstract "Thylakoid membranes of Chlamydomonas reinhardtii were fractionated into appressed membranes and non-appressed vesicles. The latter had a Chl a/b ratio of 3.2 and contained 1.12 mmol PS II (mol Chl a+b)−1. The PS II centers in the non-appressed regions displayed the kinetic characteristics of PS IIβ centers. They were able to generate a stable charge-separation to QA, but they were unable to transfer electrons from Q−A to QB (QB-nonreducing centers). After a strong-irradiance treatment (SIT) of cells by exposure to 2000 μmol · m−2 · s−1 for 15 min, a portion of the PS IIα centers in the appressed membranes was damaged due to photoinhibition. PS IIβ centers were resistant to photoinhibitory damage; however, SIT caused a lowering of the PS II content in the non-appressed fraction to 0.60 mmol PS II (mol Chl a+b)−1. Concomitantly, there was a conversion of QB-nonreducing centers to a form able to reduce the plastoquinone pool (QB-reducing centers). Low-temperature during SIT inhibited both the depletion of PS IIβ from the non-appressed membrane region and the activation of QB-nonreducing centers. These results are consistent with a ‘PS II repair cycle’ which translocates PS II units from the stroma-exposed membranes into the appressed thylakoids during activation of PS IIβ from a QB-nonreducing to a QB-reducing form. Since the overall distribution of Chl-protein complexes between the stroma-exposed and appressed membrane regions was unaffected by SIT, it is suggested that damaged PS II units from the appressed membrane region are exchanged with PS IIβ from the stroma-exposed membranes." @default.
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- W1970781932 date "1991-01-01" @default.
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- W1970781932 title "Dynamics of Photosystem II heterogeneity during photoinhibition: depletion of PS IIβ from non-appressed thylakoids during strong-irradiance exposure of Chlamydomonas reinhardtii" @default.
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- W1970781932 doi "https://doi.org/10.1016/s0005-2728(05)80287-7" @default.
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