FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1970783220> ?p ?o ?g. }

• W1970783220 abstract "Kinetics of myo-inositol (MI) uptake into primary cultures of bovine corneal endothelial cells (CEC) were studied. Confluent corneal endothelial cells accumulated 3H-MI in a time dependent and saturable process. At a narrow range of external concentrations of 3H-MI (4-50 microM), the Na(+)-dependent MI uptake followed saturation kinetics. The apparent Km value was 20 microM with a maximum velocity (Vmax) of 16 pmol/20 min/micrograms DNA. At low external 3H-MI concentrations the uptake was dependent on Na ions, but at higher levels the Na(+)-independent fraction of MI uptake significantly increased. The uptake was sensitive to removal of Ca ions and to the presence of inhibitors such as n-ethyl maleimide, phlorizin, ouabain, and amiloride (an inhibitor of Na+/H+ exchanger). The sensitivity of MI uptake toward inhibitors and ionic changes in the bathing media was reduced as external concentrations of 3H-MI increased. Citrate at 0.5 mM increased the uptake, suggesting involvement of mitochondrial oxidative metabolism in the MI uptake. Percent release of radioactivity by 2 min, after an initial 40-min incubation with 20 microM 3H-MI, was 6.6% +/- 0.8 or 35% +/- 4 when release media contained BSS alone or BSS containing 5 mM nonradioactive MI, respectively. Efflux of radioactivity from the cells also was enhanced when release media contained 40 mM glucose. Glucose and galactose as well as nonmetabolizable glucose analogues, such as 3O-methyl glucose or alpha-methyl glucose, at high concentrations (40 mM), acutely (in the incubation media) or chronically (in the growth media) inhibited MI uptake into CEC, and the extent of inhibition was inversely proportional to the external levels of 3H-MI. However, glucose at lower levels (less than or equal to 10 mM) slightly increased MI uptake. These studies indicated that the uptake of MI into corneal endothelial cells was an Na(+)-dependent active process at a narrow range of external radioactive MI concentrations. Higher levels of MI were taken up by the cells via a passive diffusion mechanism, independent of carrier protein(s). Glucose influenced the uptake of MI in a complex manner. The increased MI efflux by glucose or by MI was perhaps due to the limited capacity of CEC for accumulation or compartmentalization of this or other solutes/osmolytes, a phenomenon that may be related to the role of CEC in maintenance of corneal deutergence. High glucose-induced inhibition of Na(+)-dependent MI uptake may be in part due to glucose regulation of Na+ fluxes and cell volume.(ABSTRACT TRUNCATED AT 400 WORDS)" @default.
• W1970783220 created "2016-06-24" @default.
• W1970783220 creator A5002793613 @default.
• W1970783220 date "1990-01-01" @default.
• W1970783220 modified "2023-09-27" @default.
• W1970783220 title "Kinetics ofmyo…Inositol Transport in Corneal Endothelial Cells: Diverse Effects of Sugars and Implications in Corneal Deutergence" @default.
• W1970783220 cites W102979596 @default.
• W1970783220 cites W1576632289 @default.
• W1970783220 cites W192700678 @default.
• W1970783220 cites W1954803374 @default.
• W1970783220 cites W1975805332 @default.
• W1970783220 cites W1984428004 @default.
• W1970783220 cites W1988051553 @default.
• W1970783220 cites W1992106353 @default.
• W1970783220 cites W2000722681 @default.
• W1970783220 cites W2001761867 @default.
• W1970783220 cites W2013955069 @default.
• W1970783220 cites W2014621910 @default.
• W1970783220 cites W2027704731 @default.
• W1970783220 cites W2029616708 @default.
• W1970783220 cites W2035565266 @default.
• W1970783220 cites W2043371256 @default.
• W1970783220 cites W2044192315 @default.
• W1970783220 cites W2044574385 @default.
• W1970783220 cites W2046150411 @default.
• W1970783220 cites W2055389833 @default.
• W1970783220 cites W2060370135 @default.
• W1970783220 cites W2074062917 @default.
• W1970783220 cites W2079528637 @default.
• W1970783220 cites W2083071720 @default.
• W1970783220 cites W2092939353 @default.
• W1970783220 cites W2101715097 @default.
• W1970783220 cites W2107079232 @default.
• W1970783220 cites W2313139849 @default.
• W1970783220 cites W2461080251 @default.
• W1970783220 cites W9543227 @default.
• W1970783220 doi "https://doi.org/10.3109/09687689009025832" @default.
• W1970783220 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2103937" @default.
• W1970783220 hasPublicationYear "1990" @default.
• W1970783220 type Work @default.
• W1970783220 sameAs 1970783220 @default.
• W1970783220 citedByCount "5" @default.
• W1970783220 crossrefType "journal-article" @default.
• W1970783220 hasAuthorship W1970783220A5002793613 @default.
• W1970783220 hasConcept C121332964 @default.
• W1970783220 hasConcept C12554922 @default.
• W1970783220 hasConcept C134018914 @default.
• W1970783220 hasConcept C148898269 @default.
• W1970783220 hasConcept C161573976 @default.
• W1970783220 hasConcept C178790620 @default.
• W1970783220 hasConcept C185592680 @default.
• W1970783220 hasConcept C188053792 @default.
• W1970783220 hasConcept C25642318 @default.
• W1970783220 hasConcept C2777866211 @default.
• W1970783220 hasConcept C2778117581 @default.
• W1970783220 hasConcept C2779133857 @default.
• W1970783220 hasConcept C2779306644 @default.
• W1970783220 hasConcept C2779608910 @default.
• W1970783220 hasConcept C537181965 @default.
• W1970783220 hasConcept C55493867 @default.
• W1970783220 hasConcept C62231903 @default.
• W1970783220 hasConcept C62520636 @default.
• W1970783220 hasConcept C86803240 @default.
• W1970783220 hasConceptScore W1970783220C121332964 @default.
• W1970783220 hasConceptScore W1970783220C12554922 @default.
• W1970783220 hasConceptScore W1970783220C134018914 @default.
• W1970783220 hasConceptScore W1970783220C148898269 @default.
• W1970783220 hasConceptScore W1970783220C161573976 @default.
• W1970783220 hasConceptScore W1970783220C178790620 @default.
• W1970783220 hasConceptScore W1970783220C185592680 @default.
• W1970783220 hasConceptScore W1970783220C188053792 @default.
• W1970783220 hasConceptScore W1970783220C25642318 @default.
• W1970783220 hasConceptScore W1970783220C2777866211 @default.
• W1970783220 hasConceptScore W1970783220C2778117581 @default.
• W1970783220 hasConceptScore W1970783220C2779133857 @default.
• W1970783220 hasConceptScore W1970783220C2779306644 @default.
• W1970783220 hasConceptScore W1970783220C2779608910 @default.
• W1970783220 hasConceptScore W1970783220C537181965 @default.
• W1970783220 hasConceptScore W1970783220C55493867 @default.
• W1970783220 hasConceptScore W1970783220C62231903 @default.
• W1970783220 hasConceptScore W1970783220C62520636 @default.
• W1970783220 hasConceptScore W1970783220C86803240 @default.
• W1970783220 hasLocation W19707832201 @default.
• W1970783220 hasLocation W19707832202 @default.
• W1970783220 hasOpenAccess W1970783220 @default.
• W1970783220 hasPrimaryLocation W19707832201 @default.
• W1970783220 hasRelatedWork W1973163595 @default.
• W1970783220 hasRelatedWork W1989170258 @default.
• W1970783220 hasRelatedWork W2037166586 @default.
• W1970783220 hasRelatedWork W2062228869 @default.
• W1970783220 hasRelatedWork W2082821830 @default.
• W1970783220 hasRelatedWork W2123745710 @default.
• W1970783220 hasRelatedWork W2398005599 @default.
• W1970783220 hasRelatedWork W2416630674 @default.
• W1970783220 hasRelatedWork W3109074983 @default.
• W1970783220 hasRelatedWork W95909497 @default.
• W1970783220 isParatext "false" @default.
• W1970783220 isRetracted "false" @default.
• W1970783220 magId "1970783220" @default.
• W1970783220 workType "article" @default.