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- W1970785320 abstract "Multiple ligand-binding properties of the 30-kDa chicken alpha(1)-acid glycoprotein (cAGP), a member of the lipocalin protein family, were investigated for the first time by using circular dichroism (CD) and UV/Vis absorption spectroscopy methods. By measuring induced CD (ICD) spectra, high-affinity binding (K(a) approximately 10(5)-10(6) M(-1)) of several drugs, dyes and natural compounds to cAGP was demonstrated including antimalarial agents (quinacrine, primaquine), phenotiazines (chlorpromazine, methylene blue), propranolol, non-steroidal antiinflammatory drugs (ketoprofen, diclofenac), tamoxifen, diazepam, tacrine, dicoumarol, cationic dyes (auramine O, thioflavine T, ethidium bromide), benzo[a]pyrene, L-thyroxine, bile pigments (bilirubin, biliverdin), alkaloids (piperine, aristolochic acid), saturated and unsaturated fatty acids. Analysis of the extrinsic CD spectra with the study of the covalently modified protein and CD displacement experiments revealed that a single Trp26 residue of cAGP conserved in the whole lipocalin family is part of the binding site, and it is essentially involved in the ligand-binding process via pi-pi stacking interaction resulting in the appearance of strong induced CD bands due to the non-degenerate intermolecular exciton coupling between the pi-pi* transitions of the stacked indole ring-ligand chromophore. The finding that cAGP is able to accommodate a broad spectrum of ligands belonging to different chemical classes suggests that its core beta-barrel cavity is unusually wide containing overlapping sub-sites. Significance of these new data in understanding of the ligand-binding properties of other lipocalins, especially that of human AGP, and potential practical applications are briefly discussed. Overall, cAGP serves as a simple, ultimate model to extend our knowledge on ligand-binding properties of lipocalins and to study the role of tryptophan residues in molecular recognition processes." @default.
- W1970785320 created "2016-06-24" @default.
- W1970785320 creator A5016901583 @default.
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- W1970785320 creator A5055019777 @default.
- W1970785320 date "2006-08-01" @default.
- W1970785320 modified "2023-10-01" @default.
- W1970785320 title "Multiple ligand-binding properties of the lipocalin member chicken α1-acid glycoprotein studied by circular dichroism and electronic absorption spectroscopy: The essential role of the conserved tryptophan residue" @default.
- W1970785320 cites W14366731 @default.
- W1970785320 cites W1482086331 @default.
- W1970785320 cites W1532217875 @default.
- W1970785320 cites W1617965928 @default.
- W1970785320 cites W1806881405 @default.
- W1970785320 cites W1942540475 @default.
- W1970785320 cites W1965859012 @default.
- W1970785320 cites W1966233011 @default.
- W1970785320 cites W1968586979 @default.
- W1970785320 cites W1971257890 @default.
- W1970785320 cites W1977064549 @default.
- W1970785320 cites W1977147294 @default.
- W1970785320 cites W1978504593 @default.
- W1970785320 cites W1988146892 @default.
- W1970785320 cites W1989012571 @default.
- W1970785320 cites W1989270637 @default.
- W1970785320 cites W1989536432 @default.
- W1970785320 cites W1993063963 @default.
- W1970785320 cites W1993412848 @default.
- W1970785320 cites W1994708463 @default.
- W1970785320 cites W1994790663 @default.
- W1970785320 cites W1996453536 @default.
- W1970785320 cites W1998374689 @default.
- W1970785320 cites W2000455214 @default.
- W1970785320 cites W2001988330 @default.
- W1970785320 cites W2003414607 @default.
- W1970785320 cites W2004929427 @default.
- W1970785320 cites W2005518727 @default.
- W1970785320 cites W2010193505 @default.
- W1970785320 cites W2012666484 @default.
- W1970785320 cites W2012719485 @default.
- W1970785320 cites W2020014392 @default.
- W1970785320 cites W2021518709 @default.
- W1970785320 cites W2022015453 @default.
- W1970785320 cites W2025584407 @default.
- W1970785320 cites W2030422668 @default.
- W1970785320 cites W2032449042 @default.
- W1970785320 cites W2032713792 @default.
- W1970785320 cites W2035043090 @default.
- W1970785320 cites W2035267033 @default.
- W1970785320 cites W2037275316 @default.
- W1970785320 cites W2038438679 @default.
- W1970785320 cites W2039873895 @default.
- W1970785320 cites W2040141270 @default.
- W1970785320 cites W2043936968 @default.
- W1970785320 cites W2046140230 @default.
- W1970785320 cites W2050608687 @default.
- W1970785320 cites W2051457523 @default.
- W1970785320 cites W2053430706 @default.
- W1970785320 cites W2055973543 @default.
- W1970785320 cites W2057080881 @default.
- W1970785320 cites W2058151768 @default.
- W1970785320 cites W2063652106 @default.
- W1970785320 cites W2066767743 @default.
- W1970785320 cites W2068937695 @default.
- W1970785320 cites W2069201530 @default.
- W1970785320 cites W2069608411 @default.
- W1970785320 cites W2071692690 @default.
- W1970785320 cites W2079316046 @default.
- W1970785320 cites W2081140858 @default.
- W1970785320 cites W2081969998 @default.
- W1970785320 cites W2090255819 @default.
- W1970785320 cites W2090286141 @default.
- W1970785320 cites W2103311340 @default.
- W1970785320 cites W2104017463 @default.
- W1970785320 cites W2116677499 @default.
- W1970785320 cites W2121093342 @default.
- W1970785320 cites W2126147415 @default.
- W1970785320 cites W2128329009 @default.
- W1970785320 cites W2130441976 @default.
- W1970785320 cites W2138498116 @default.
- W1970785320 cites W2139720385 @default.
- W1970785320 cites W2142529984 @default.
- W1970785320 cites W2146792913 @default.
- W1970785320 cites W2158314083 @default.
- W1970785320 cites W2159829830 @default.
- W1970785320 cites W2162463986 @default.
- W1970785320 cites W2209250776 @default.
- W1970785320 cites W2329353164 @default.
- W1970785320 cites W2950459289 @default.
- W1970785320 cites W2950798492 @default.
- W1970785320 cites W2952027704 @default.
- W1970785320 cites W2953157016 @default.
- W1970785320 cites W4232239100 @default.
- W1970785320 doi "https://doi.org/10.1016/j.bbagen.2006.04.006" @default.
- W1970785320 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16813999" @default.
- W1970785320 hasPublicationYear "2006" @default.
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