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• W1970785921 abstract "Accessory cells such as macrophages and natural killer cells, and their cytokines such as IL-10, IL-12, and IFN-gamma have been suggested to play a critical role in the development of T helper cells.Both natural killer cells and peripheral blood mononuclear cells were isolated and stimulated for their ability in producing cytokines. In addition, the percentage of IFN-gamma-secreting cells was analyzed with the method of intracellular staining.The data suggested (1) no significant difference between asthmatic children and normal controls in number, cytotoxicity, and IFN-gamma production of purified NK cells; (2) decreased secretion of IL-12 by stimulated peripheral blood mononuclear cells in asthmatic children compared with normals (P < .05); (3) decreased production of IFN-gamma by PBMC from asthmatic children compared with normals (P < .05); and (4) intracellular expressed IFN-gamma level was lower in CD4+ T cells of asthmatic children (P < .05).The results suggested that IL-12 produced predominantly by macrophages and associated decreased IFN-gamma-secreting CD4+ T cells play a critical role in the pathogenesis of asthma." @default.
• W1970785921 created "2016-06-24" @default.
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• W1970785921 date "1999-05-01" @default.
• W1970785921 modified "2023-10-14" @default.
• W1970785921 title "Reduced IL-12 level correlates with decreased IFN-γ secreting T cells but not natural killer cell activity in asthmatic children" @default.
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• W1970785921 doi "https://doi.org/10.1016/s1081-1206(10)62725-1" @default.
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