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- W1970786020 abstract "The bovine oxytocin precursor was expressed in Escherichia coli as a fusion protein by cloning the hormone encoding cDNA in frame behind the replicase gene of the RNA phage MS2. By step-wise extraction with different urea concentrations, the fusion protein was enriched in the 7 M urea fraction and further purified by Sephacryl S-300 chromatography. The oxytocin precursor was cleaved off the fusion protein by cyanogen bromide treatment, chromatographed on FPLC columns and identified by Western blot analysis, using antibodies raised against neurophysin." @default.
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- W1970786020 date "1988-01-01" @default.
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- W1970786020 title "Expression of the bovine hypothalamic hormone oxytocin precursor in Escherichia coli" @default.
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- W1970786020 doi "https://doi.org/10.1016/0300-9084(88)90165-4" @default.
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