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- W1970804954 abstract "Developmental stages of the flagellate Blastocrithidia triatomae were isolated according to differences in density or in surface charge from dry feces or the intestinal content of reduviid hosts or from in vitro cultures which contained host cells. Isopycnic ultracentrifugation on isoosmotic Percoll gradients separated cysts (ϱ = 1.145 g/cm3) from epimastigotes (ϱ = 1.075 g/cm3), representing the two major morphological stages of B. triatomae. Moreover, a continuous shift in buoyant density of encystating and/or excystating stages from the level of mature cysts to lower densities displayed by epimastigotes was observed. This characteristic excluded the isolation of pure epimastigotes by density gradients. Nevertheless, approximately 1 × 108 mature cysts per gradient were obtained by this procedure. Employing DEAE-ion exchange chromatography for cyst isolation, approximately 2 × 1010 cysts could be purified per column. In addition, DEAE-chromatography was used for the isolation of pure epimastigote fractions. The succesful isolation of B. triatomae-stages from three completely different sources suggests that the methods derived in our study may be easily adapted for the isolation of other insect trypanosomatids." @default.
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- W1970804954 date "1988-04-01" @default.
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- W1970804954 title "Isolation and separation of cysts and epimastigotes of Blastocrithidia triatomae Cerisola et al., 1971 (Trypanosomatidae)" @default.
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- W1970804954 doi "https://doi.org/10.1016/0167-7012(88)90022-x" @default.
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