FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1970840027> ?p ?o ?g. }

• W1970840027 endingPage "1753" @default.
• W1970840027 startingPage "1744" @default.
• W1970840027 abstract "ABSTRACT Pseudomonas fluorescens is an efficient platform for recombinant protein production. P. fluorescens has an ABC transporter secreting endogenous thermostable lipase (TliA) and protease, which can be exploited to transport recombinant proteins across the cell membrane. In this study, the expression vector pDART was constructed by inserting tliDEF , genes encoding the ABC transporter, along with the construct of the lipase ABC transporter recognition domain (LARD), into pDSK519, a widely used shuttle vector. When the gene for the target protein was inserted into the vector, the C-terminally fused LARD allowed it to be secreted through the ABC transporter into the extracellular medium. After secretion of the fused target protein, the LARD containing a hydrophobic C terminus enabled its purification through hydrophobic interaction chromatography (HIC) using a methyl-Sepharose column. Alkaline phosphatase (AP) and green fluorescent protein (GFP) were used to validate the expression, export, and purification of target proteins by the pDART system. Both proteins were secreted into the extracellular medium in P. fluorescens . In particular, AP was secreted in several Pseudomonas species with its enzymatic activity in extracellular media. Furthermore, purification of the target protein using HIC yielded some degree of AP and GFP purification, where AP was purified to almost a single product. The pDART system will provide greater convenience for the secretory production and purification of recombinant proteins in Gram-negative bacteria, such as Pseudomonas species." @default.
• W1970840027 created "2016-06-24" @default.
• W1970840027 creator A5009370266 @default.
• W1970840027 creator A5049933150 @default.
• W1970840027 creator A5050315100 @default.
• W1970840027 creator A5062746746 @default.
• W1970840027 creator A5065717580 @default.
• W1970840027 date "2015-03-01" @default.
• W1970840027 modified "2023-09-30" @default.
• W1970840027 title "A Vector System for ABC Transporter-Mediated Secretion and Purification of Recombinant Proteins in Pseudomonas Species" @default.
• W1970840027 cites W1516116810 @default.
• W1970840027 cites W1567346833 @default.
• W1970840027 cites W1582760489 @default.
• W1970840027 cites W1620632095 @default.
• W1970840027 cites W1887601749 @default.
• W1970840027 cites W1892148740 @default.
• W1970840027 cites W1894220132 @default.
• W1970840027 cites W1972010400 @default.
• W1970840027 cites W1975304761 @default.
• W1970840027 cites W1983219090 @default.
• W1970840027 cites W1992764122 @default.
• W1970840027 cites W2003385479 @default.
• W1970840027 cites W2022959668 @default.
• W1970840027 cites W2030669640 @default.
• W1970840027 cites W2039308194 @default.
• W1970840027 cites W2041918990 @default.
• W1970840027 cites W2046608295 @default.
• W1970840027 cites W2048940219 @default.
• W1970840027 cites W2049322319 @default.
• W1970840027 cites W2053152876 @default.
• W1970840027 cites W2056399792 @default.
• W1970840027 cites W2064788965 @default.
• W1970840027 cites W2067326140 @default.
• W1970840027 cites W2075366305 @default.
• W1970840027 cites W2082047115 @default.
• W1970840027 cites W2085338944 @default.
• W1970840027 cites W2092609221 @default.
• W1970840027 cites W2094250868 @default.
• W1970840027 cites W2097899215 @default.
• W1970840027 cites W2098347362 @default.
• W1970840027 cites W2099673165 @default.
• W1970840027 cites W2106246537 @default.
• W1970840027 cites W2109990053 @default.
• W1970840027 cites W2120293095 @default.
• W1970840027 cites W2130693454 @default.
• W1970840027 cites W2138984785 @default.
• W1970840027 cites W2139543196 @default.
• W1970840027 cites W2143948627 @default.
• W1970840027 cites W2151346312 @default.
• W1970840027 cites W2160840048 @default.
• W1970840027 cites W2172154503 @default.
• W1970840027 cites W4239460220 @default.
• W1970840027 cites W4256017034 @default.
• W1970840027 doi "https://doi.org/10.1128/aem.03514-14" @default.
• W1970840027 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4325144" @default.
• W1970840027 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25548043" @default.
• W1970840027 hasPublicationYear "2015" @default.
• W1970840027 type Work @default.
• W1970840027 sameAs 1970840027 @default.
• W1970840027 citedByCount "15" @default.
• W1970840027 countsByYear W19708400272016 @default.
• W1970840027 countsByYear W19708400272017 @default.
• W1970840027 countsByYear W19708400272018 @default.
• W1970840027 countsByYear W19708400272019 @default.
• W1970840027 countsByYear W19708400272020 @default.
• W1970840027 countsByYear W19708400272021 @default.
• W1970840027 countsByYear W19708400272022 @default.
• W1970840027 crossrefType "journal-article" @default.
• W1970840027 hasAuthorship W1970840027A5009370266 @default.
• W1970840027 hasAuthorship W1970840027A5049933150 @default.
• W1970840027 hasAuthorship W1970840027A5050315100 @default.
• W1970840027 hasAuthorship W1970840027A5062746746 @default.
• W1970840027 hasAuthorship W1970840027A5065717580 @default.
• W1970840027 hasBestOaLocation W19708400271 @default.
• W1970840027 hasConcept C104317684 @default.
• W1970840027 hasConcept C142613039 @default.
• W1970840027 hasConcept C145640855 @default.
• W1970840027 hasConcept C149011108 @default.
• W1970840027 hasConcept C156399914 @default.
• W1970840027 hasConcept C181199279 @default.
• W1970840027 hasConcept C2776835041 @default.
• W1970840027 hasConcept C2779697368 @default.
• W1970840027 hasConcept C2781463711 @default.
• W1970840027 hasConcept C28406088 @default.
• W1970840027 hasConcept C33161422 @default.
• W1970840027 hasConcept C40767141 @default.
• W1970840027 hasConcept C44312359 @default.
• W1970840027 hasConcept C49039625 @default.
• W1970840027 hasConcept C523546767 @default.
• W1970840027 hasConcept C54355233 @default.
• W1970840027 hasConcept C55493867 @default.
• W1970840027 hasConcept C86803240 @default.
• W1970840027 hasConcept C92087593 @default.
• W1970840027 hasConceptScore W1970840027C104317684 @default.
• W1970840027 hasConceptScore W1970840027C142613039 @default.
• W1970840027 hasConceptScore W1970840027C145640855 @default.
• W1970840027 hasConceptScore W1970840027C149011108 @default.
• W1970840027 hasConceptScore W1970840027C156399914 @default.

• next