Matches in SemOpenAlex for { <https://semopenalex.org/work/W1970887682> ?p ?o ?g. }
Showing items 1 to 59 of
59
with 100 items per page.
- W1970887682 endingPage "373a" @default.
- W1970887682 startingPage "373a" @default.
- W1970887682 abstract "Kinesin is known as a dimeric motor protein, which carries cellular cargoes along microtubules by hydrolyzing ATP. Calmodulin (CaM) is a calcium binding protein that participates in cellular regulatory processes. CaM undergoes a conformational change upon binding to calcium, which enables it to bind to specific proteins for a specific response. We have previously demonstrated that kinesin fused with CaM at the C-terminal binds reversibly to M13-Qdots in a calcium dependent manner. In this study, we have tried to make the calcium dependent reversible dimerization of kinesin utilizing CaM- target peptide M13 binding system in order to control motility of kinesin. First we have designed and prepared the cDNA of the truncated kinesin (355 amino acids) that does not form dimer. Subsequently we prepared the cDNA encoding two kinesin chimeric proteins in which C-terminal of kinesin355 was fused with calmodulin (kinesin355-CaM) and fused with M13-GFP (Kinesin355-M13-GFP). The cDNAs of the kinesin chimeras were cloned into expression vector pET21a and transformed into E.coli BL21. The kinesin chimeras were successfully expressed and purified by Co-Chelate column. These kinesin chimeras showed normal ATPase activities. Furthermore, K355-CaM bound to M13-YFP in a calcium dependent manner. And the calcium dependent interaction between kinesin355-CaM and kinesin355-M13-GFP was examined." @default.
- W1970887682 created "2016-06-24" @default.
- W1970887682 creator A5014240620 @default.
- W1970887682 creator A5030307183 @default.
- W1970887682 creator A5039322459 @default.
- W1970887682 date "2010-01-01" @default.
- W1970887682 modified "2023-10-18" @default.
- W1970887682 title "Calcium Dependent Regulation of Kinesin Function using Binding System of CaM and M13 Peptide" @default.
- W1970887682 doi "https://doi.org/10.1016/j.bpj.2009.12.2012" @default.
- W1970887682 hasPublicationYear "2010" @default.
- W1970887682 type Work @default.
- W1970887682 sameAs 1970887682 @default.
- W1970887682 citedByCount "0" @default.
- W1970887682 crossrefType "journal-article" @default.
- W1970887682 hasAuthorship W1970887682A5014240620 @default.
- W1970887682 hasAuthorship W1970887682A5030307183 @default.
- W1970887682 hasAuthorship W1970887682A5039322459 @default.
- W1970887682 hasBestOaLocation W19708876821 @default.
- W1970887682 hasConcept C12554922 @default.
- W1970887682 hasConcept C178790620 @default.
- W1970887682 hasConcept C185592680 @default.
- W1970887682 hasConcept C20418707 @default.
- W1970887682 hasConcept C29688787 @default.
- W1970887682 hasConcept C519063684 @default.
- W1970887682 hasConcept C55493867 @default.
- W1970887682 hasConcept C59006786 @default.
- W1970887682 hasConcept C86803240 @default.
- W1970887682 hasConcept C95444343 @default.
- W1970887682 hasConceptScore W1970887682C12554922 @default.
- W1970887682 hasConceptScore W1970887682C178790620 @default.
- W1970887682 hasConceptScore W1970887682C185592680 @default.
- W1970887682 hasConceptScore W1970887682C20418707 @default.
- W1970887682 hasConceptScore W1970887682C29688787 @default.
- W1970887682 hasConceptScore W1970887682C519063684 @default.
- W1970887682 hasConceptScore W1970887682C55493867 @default.
- W1970887682 hasConceptScore W1970887682C59006786 @default.
- W1970887682 hasConceptScore W1970887682C86803240 @default.
- W1970887682 hasConceptScore W1970887682C95444343 @default.
- W1970887682 hasIssue "3" @default.
- W1970887682 hasLocation W19708876821 @default.
- W1970887682 hasOpenAccess W1970887682 @default.
- W1970887682 hasPrimaryLocation W19708876821 @default.
- W1970887682 hasRelatedWork W1965776429 @default.
- W1970887682 hasRelatedWork W2017522528 @default.
- W1970887682 hasRelatedWork W2025337933 @default.
- W1970887682 hasRelatedWork W2053797302 @default.
- W1970887682 hasRelatedWork W2092897706 @default.
- W1970887682 hasRelatedWork W2092964883 @default.
- W1970887682 hasRelatedWork W2143472220 @default.
- W1970887682 hasRelatedWork W2159088296 @default.
- W1970887682 hasRelatedWork W2762659278 @default.
- W1970887682 hasRelatedWork W2410580908 @default.
- W1970887682 hasVolume "98" @default.
- W1970887682 isParatext "false" @default.
- W1970887682 isRetracted "false" @default.
- W1970887682 magId "1970887682" @default.
- W1970887682 workType "article" @default.