FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1970969912> ?p ?o ?g. }

• W1970969912 endingPage "80" @default.
• W1970969912 startingPage "71" @default.
• W1970969912 abstract "Human amniotic fluid stem cells (AFSCs) are emerging as an important cell source for tissue engineering and regenerative medicine due to their easy accessibility and broad multi-potentiality. In clinical applications, a large number of human AFSCs are required, which cannot be provided in conventional 2-dimensional (2-D) culture systems. To address this issue, the expansion of human AFSCs in 3-dimensional (3-D) polyethylene terephthalate (PET) scaffolds in a stirred bioreactor was evaluated. The results showed that 3-D PET scaffold with in vivo-like environment and a large specific surface area for cell adhesion promoted cell expansion (66-fold vs. 38-fold) compared to 2-D culture. A dynamic fibrous bed bioreactor (FBB) was used to expand AFSCs to reach a high cell density of 3.2 × 106 cells/mL. The bioreactor-expanded cells maintained clonogenic ability and high levels of expression (95.5–99.8%) of characteristic stem cell surface makers, including CD29, CD44, CD90 and CD105. The differentiation of bioreactor-expanded AFSCs into osteogenic and adipogeneic lineages was demonstrated with Alizarin red S and Oil Red O staining, respectively, and further confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) analysis. This study demonstrated the feasibility of using the FBB to mass-produce human AFSCs for potential applications in tissue engineering and regenerative medicine." @default.
• W1970969912 created "2016-06-24" @default.
• W1970969912 creator A5001465349 @default.
• W1970969912 creator A5052052129 @default.
• W1970969912 creator A5062842171 @default.
• W1970969912 date "2014-01-01" @default.
• W1970969912 modified "2023-10-06" @default.
• W1970969912 title "Expansion of human amniotic fluid stem cells in 3-dimensional fibrous scaffolds in a stirred bioreactor" @default.
• W1970969912 cites W1834815540 @default.
• W1970969912 cites W1964060266 @default.
• W1970969912 cites W1964324925 @default.
• W1970969912 cites W1969110579 @default.
• W1970969912 cites W1974462554 @default.
• W1970969912 cites W1974915473 @default.
• W1970969912 cites W1978603428 @default.
• W1970969912 cites W1979737622 @default.
• W1970969912 cites W1980776838 @default.
• W1970969912 cites W2000721992 @default.
• W1970969912 cites W2004000166 @default.
• W1970969912 cites W2005848913 @default.
• W1970969912 cites W2013158875 @default.
• W1970969912 cites W2013443778 @default.
• W1970969912 cites W2015101509 @default.
• W1970969912 cites W2016931709 @default.
• W1970969912 cites W2017655153 @default.
• W1970969912 cites W2018013954 @default.
• W1970969912 cites W2025262230 @default.
• W1970969912 cites W2027034506 @default.
• W1970969912 cites W2028876849 @default.
• W1970969912 cites W2034789248 @default.
• W1970969912 cites W2035815508 @default.
• W1970969912 cites W2036348189 @default.
• W1970969912 cites W2037237480 @default.
• W1970969912 cites W2041703442 @default.
• W1970969912 cites W2045456080 @default.
• W1970969912 cites W2054134839 @default.
• W1970969912 cites W2061617750 @default.
• W1970969912 cites W2063265877 @default.
• W1970969912 cites W2071536607 @default.
• W1970969912 cites W2078266417 @default.
• W1970969912 cites W2082781093 @default.
• W1970969912 cites W2092364499 @default.
• W1970969912 cites W2101967659 @default.
• W1970969912 cites W2107649335 @default.
• W1970969912 cites W2117925405 @default.
• W1970969912 cites W2132875813 @default.
• W1970969912 cites W2136833516 @default.
• W1970969912 cites W2141480483 @default.
• W1970969912 cites W2159999899 @default.
• W1970969912 cites W2165438231 @default.
• W1970969912 doi "https://doi.org/10.1016/j.bej.2013.11.001" @default.
• W1970969912 hasPublicationYear "2014" @default.
• W1970969912 type Work @default.
• W1970969912 sameAs 1970969912 @default.
• W1970969912 citedByCount "10" @default.
• W1970969912 countsByYear W19709699122014 @default.
• W1970969912 countsByYear W19709699122016 @default.
• W1970969912 countsByYear W19709699122017 @default.
• W1970969912 countsByYear W19709699122018 @default.
• W1970969912 countsByYear W19709699122019 @default.
• W1970969912 crossrefType "journal-article" @default.
• W1970969912 hasAuthorship W1970969912A5001465349 @default.
• W1970969912 hasAuthorship W1970969912A5052052129 @default.
• W1970969912 hasAuthorship W1970969912A5062842171 @default.
• W1970969912 hasConcept C10205521 @default.
• W1970969912 hasConcept C10854531 @default.
• W1970969912 hasConcept C116716535 @default.
• W1970969912 hasConcept C136229726 @default.
• W1970969912 hasConcept C1491633281 @default.
• W1970969912 hasConcept C168170006 @default.
• W1970969912 hasConcept C178790620 @default.
• W1970969912 hasConcept C185592680 @default.
• W1970969912 hasConcept C2780932548 @default.
• W1970969912 hasConcept C28328180 @default.
• W1970969912 hasConcept C49892992 @default.
• W1970969912 hasConcept C55493867 @default.
• W1970969912 hasConcept C71924100 @default.
• W1970969912 hasConcept C86803240 @default.
• W1970969912 hasConcept C95444343 @default.
• W1970969912 hasConceptScore W1970969912C10205521 @default.
• W1970969912 hasConceptScore W1970969912C10854531 @default.
• W1970969912 hasConceptScore W1970969912C116716535 @default.
• W1970969912 hasConceptScore W1970969912C136229726 @default.
• W1970969912 hasConceptScore W1970969912C1491633281 @default.
• W1970969912 hasConceptScore W1970969912C168170006 @default.
• W1970969912 hasConceptScore W1970969912C178790620 @default.
• W1970969912 hasConceptScore W1970969912C185592680 @default.
• W1970969912 hasConceptScore W1970969912C2780932548 @default.
• W1970969912 hasConceptScore W1970969912C28328180 @default.
• W1970969912 hasConceptScore W1970969912C49892992 @default.
• W1970969912 hasConceptScore W1970969912C55493867 @default.
• W1970969912 hasConceptScore W1970969912C71924100 @default.
• W1970969912 hasConceptScore W1970969912C86803240 @default.
• W1970969912 hasConceptScore W1970969912C95444343 @default.
• W1970969912 hasLocation W19709699121 @default.
• W1970969912 hasOpenAccess W1970969912 @default.
• W1970969912 hasPrimaryLocation W19709699121 @default.
• W1970969912 hasRelatedWork W1982582541 @default.

• next