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- W1971012945 abstract "We investigated the origins of greater clot rigidity associated with FXIIIa-dependent cross-linking. Fibrin clots were examined in which cross-linking was controlled through the use of two inhibitors: a highly specific active-center-directed synthetic inhibitor of FXIIIa, 1,3-dimethyl-4,5-diphenyl-2[2(oxopropyl)thio]imidazolium trifluoromethylsulfonate, and a patient-derived immunoglobulin directed mainly against the thrombin-activated catalytic A subunits of thrombin-activated FXIII. Cross-linked fibrin chains were identified and quantified by one- and two-dimensional gel electrophoresis and immunostaining with antibodies specific for the alpha- and gamma-chains of fibrin. Gamma-dimers, gamma-multimers, alpha(n)-polymers, and alpha(p)gamma(q)-hybrids were detected. The synthetic inhibitor was highly effective in preventing the production of all cross-linked species. In contrast, the autoimmune antibody of the patient caused primarily an inhibition of alpha-chain cross-linking. Clot rigidities (storage moduli, G') were measured with a cone and plate rheometer and correlated with the distributions of the various cross-linked species found in the clots. Our findings indicate that the FXIIIa-induced dimeric cross-linking of gamma-chains by itself is not sufficient to stiffen the fibrin networks. Instead, the augmentation of clot rigidity was more strongly correlated with the formation of gamma-multimers, alpha(n)-polymers, and alpha(p)gamma(q)-hybrid cross-links. A mechanism is proposed to explain how these cross-linked species may enhance clot rigidity." @default.
- W1971012945 created "2016-06-24" @default.
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- W1971012945 date "1999-11-01" @default.
- W1971012945 modified "2023-10-05" @default.
- W1971012945 title "Influence of a Natural and a Synthetic Inhibitor of Factor XIIIa on Fibrin Clot Rheology" @default.
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- W1971012945 cites W1533436380 @default.
- W1971012945 cites W1536945368 @default.
- W1971012945 cites W1538720735 @default.
- W1971012945 cites W1552506639 @default.
- W1971012945 cites W1556587647 @default.
- W1971012945 cites W1563363176 @default.
- W1971012945 cites W1568462251 @default.
- W1971012945 cites W1587148305 @default.
- W1971012945 cites W1606423922 @default.
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- W1971012945 cites W1981438065 @default.
- W1971012945 cites W1986651104 @default.
- W1971012945 cites W1987532483 @default.
- W1971012945 cites W1990853221 @default.
- W1971012945 cites W1994542189 @default.
- W1971012945 cites W1995967683 @default.
- W1971012945 cites W1996199589 @default.
- W1971012945 cites W1997614271 @default.
- W1971012945 cites W1999504108 @default.
- W1971012945 cites W2001922591 @default.
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- W1971012945 cites W2022143868 @default.
- W1971012945 cites W2024664270 @default.
- W1971012945 cites W2025358191 @default.
- W1971012945 cites W2026648151 @default.
- W1971012945 cites W2028047806 @default.
- W1971012945 cites W2028073085 @default.
- W1971012945 cites W2029405287 @default.
- W1971012945 cites W2030935317 @default.
- W1971012945 cites W2033233335 @default.
- W1971012945 cites W2034549620 @default.
- W1971012945 cites W2037068857 @default.
- W1971012945 cites W2042894685 @default.
- W1971012945 cites W2043294207 @default.
- W1971012945 cites W2045202161 @default.
- W1971012945 cites W2045529021 @default.
- W1971012945 cites W2046153111 @default.
- W1971012945 cites W2051889991 @default.
- W1971012945 cites W2054260209 @default.
- W1971012945 cites W2056593430 @default.
- W1971012945 cites W2057577566 @default.
- W1971012945 cites W2059533011 @default.
- W1971012945 cites W2061246487 @default.
- W1971012945 cites W2069578485 @default.
- W1971012945 cites W2071756117 @default.
- W1971012945 cites W2076170570 @default.
- W1971012945 cites W2079235548 @default.
- W1971012945 cites W2080634703 @default.
- W1971012945 cites W2082618660 @default.
- W1971012945 cites W2085213487 @default.
- W1971012945 cites W2086056309 @default.
- W1971012945 cites W2086177318 @default.
- W1971012945 cites W2092608701 @default.
- W1971012945 cites W2100837269 @default.
- W1971012945 cites W2101108802 @default.
- W1971012945 cites W2111956080 @default.
- W1971012945 cites W2124538268 @default.
- W1971012945 cites W2394863100 @default.
- W1971012945 cites W2397837283 @default.
- W1971012945 cites W2399458495 @default.
- W1971012945 cites W2401950536 @default.
- W1971012945 cites W2403179525 @default.
- W1971012945 cites W2403741842 @default.
- W1971012945 cites W2410349656 @default.
- W1971012945 cites W2411877862 @default.
- W1971012945 cites W2415714106 @default.
- W1971012945 cites W2417660540 @default.
- W1971012945 cites W2418161660 @default.
- W1971012945 cites W2425411105 @default.
- W1971012945 cites W2471022850 @default.
- W1971012945 cites W2473676894 @default.
- W1971012945 cites W4295154367 @default.
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- W1971012945 doi "https://doi.org/10.1016/s0006-3495(99)77114-6" @default.
- W1971012945 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1300554" @default.
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