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- W1971014750 abstract "Summary We have characterised the major circulating ecdysteroid in the South African spiny lobster, Jasus lalandii, as 20-hydroxyecdysone (20E) by means of chromatographic separation and an enzyme immunoassay (EIA). The titre of ecdysteroids in the haemolymph, as quantified by EIA, varied greatly during the moult cycle: it was highest in premoult and lowest in postmoult stages. A significant decrease in ecdysteroid levels was measured shortly before ecdysis. Since the major circulating ecdysteroid in J. lalandii is apparently 20E, which arises from peripheral 20-hydroxylation of ecdysone, we concluded that the Y-organs of this spiny lobster are synthesising ecdysone or/and 3-dehydroecdysone. In order to establish an in vitro assay with which to characterise moult-inhibiting hormone (MIH) activity in the sinus gland, we have incubated Y-organs from J. lalandii in vitro in the presence of crude extracts of homospecific sinus glands and measured the secreted ecdysteroid titres by EIA. Our experiments show th..." @default.
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- W1971014750 date "2001-07-01" @default.
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- W1971014750 title "Characterisation of moult-inhibiting activities of sinus glands of the spiny lobster,Jasus lalandii" @default.
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- W1971014750 doi "https://doi.org/10.1080/07924259.2001.9652473" @default.
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