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- W1971074642 abstract "A high-performance liquid chromatographic method with indirect fluorescence detection has been developed for quantification of dipicolinic acid, a major constituent of bacterial endospores. After separation on a reversed-phase column, a post-column reagent of sodium acetate at 1 mol l− 1 with 50 μmol l− 1 terbium chloride was added for complexation of dipicolinic acid. Terbium monodipicolinate complexes formed were quantified by measuring the fluorescence emission maximum at 548 nm after excitation with UV light at 270 nm wavelength. Parameters of post-column complexation were optimized to achieve a detection limit of 0.5 nmol DPA l− 1, corresponding to about 103 Desulfosporosinus orientis endospores per ml. The method was applied to the analysis of spore contamination in tuna and for estimating the endospore numbers in marine sediments." @default.
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- W1971074642 date "2007-08-01" @default.
- W1971074642 modified "2023-10-18" @default.
- W1971074642 title "A highly sensitive HPLC method for determination of nanomolar concentrations of dipicolinic acid, a characteristic constituent of bacterial endospores" @default.
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- W1971074642 doi "https://doi.org/10.1016/j.mimet.2007.05.008" @default.
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