FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1971086498> ?p ?o ?g. }

• W1971086498 endingPage "587" @default.
• W1971086498 startingPage "581" @default.
• W1971086498 abstract "Summary The bacterial artificial chromosome (BAC) has become the most popular tool for cloning large DNA fragments. The inserts of most BAC clones average 100–200 kilobases (kb) and molecular characterization of such large DNA fragments is a major challenge. Here we report a simple and expedient technique for physical mapping of BAC inserts. Individual BAC molecules were immobilized on glass slides coated with Poly‐L‐lysine. The intact circular BAC molecules were visualized by fluorescence in situ hybridization using BAC DNA as a probe. The 7.4 kb BAC vector was extended to approximately 2.44 kb per micrometer. Digitally measured linear distances can be transformed into kilobases of DNA using the extension of BAC vector as a standard calibration. We mapped DNA fragments as small as 2 kb directly on circular BAC molecules. A rice BAC clone containing both tandem and dispersed repeats was analyzed using this technique. The distribution and organization of the different repeats within the BAC insert were efficiently determined. The results showed that this technique will be especially valuable for characterizing BAC clones that contain complex repetitive DNA sequences." @default.
• W1971086498 created "2016-06-24" @default.
• W1971086498 creator A5014565277 @default.
• W1971086498 creator A5062535756 @default.
• W1971086498 creator A5084728323 @default.
• W1971086498 date "1999-03-01" @default.
• W1971086498 modified "2023-10-16" @default.
• W1971086498 title "Digital mapping of bacterial artificial chromosomes by fluorescence <i>in situ</i> hybridization" @default.
• W1971086498 cites W1971701163 @default.
• W1971086498 cites W1972372764 @default.
• W1971086498 cites W1976632230 @default.
• W1971086498 cites W1991713583 @default.
• W1971086498 cites W1996749102 @default.
• W1971086498 cites W1998397150 @default.
• W1971086498 cites W2015656666 @default.
• W1971086498 cites W2018107910 @default.
• W1971086498 cites W2018157600 @default.
• W1971086498 cites W2021608281 @default.
• W1971086498 cites W2029055264 @default.
• W1971086498 cites W2031117804 @default.
• W1971086498 cites W2035695102 @default.
• W1971086498 cites W2040304965 @default.
• W1971086498 cites W2060183661 @default.
• W1971086498 cites W2062960549 @default.
• W1971086498 cites W2095475303 @default.
• W1971086498 cites W2102049511 @default.
• W1971086498 cites W2107305616 @default.
• W1971086498 cites W2109352113 @default.
• W1971086498 cites W2161728879 @default.
• W1971086498 cites W2172164880 @default.
• W1971086498 cites W4245694358 @default.
• W1971086498 doi "https://doi.org/10.1046/j.1365-313x.1999.00398.x" @default.
• W1971086498 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10205912" @default.
• W1971086498 hasPublicationYear "1999" @default.
• W1971086498 type Work @default.
• W1971086498 sameAs 1971086498 @default.
• W1971086498 citedByCount "35" @default.
• W1971086498 countsByYear W19710864982012 @default.
• W1971086498 countsByYear W19710864982013 @default.
• W1971086498 countsByYear W19710864982015 @default.
• W1971086498 countsByYear W19710864982016 @default.
• W1971086498 countsByYear W19710864982018 @default.
• W1971086498 countsByYear W19710864982019 @default.
• W1971086498 countsByYear W19710864982023 @default.
• W1971086498 crossrefType "journal-article" @default.
• W1971086498 hasAuthorship W1971086498A5014565277 @default.
• W1971086498 hasAuthorship W1971086498A5062535756 @default.
• W1971086498 hasAuthorship W1971086498A5084728323 @default.
• W1971086498 hasBestOaLocation W19710864981 @default.
• W1971086498 hasConcept C104317684 @default.
• W1971086498 hasConcept C107618985 @default.
• W1971086498 hasConcept C127413603 @default.
• W1971086498 hasConcept C130197536 @default.
• W1971086498 hasConcept C13575015 @default.
• W1971086498 hasConcept C141231307 @default.
• W1971086498 hasConcept C1436000 @default.
• W1971086498 hasConcept C153911025 @default.
• W1971086498 hasConcept C168374021 @default.
• W1971086498 hasConcept C182901222 @default.
• W1971086498 hasConcept C187882448 @default.
• W1971086498 hasConcept C19924922 @default.
• W1971086498 hasConcept C2780098074 @default.
• W1971086498 hasConcept C3017666073 @default.
• W1971086498 hasConcept C40767141 @default.
• W1971086498 hasConcept C42062724 @default.
• W1971086498 hasConcept C523546767 @default.
• W1971086498 hasConcept C54355233 @default.
• W1971086498 hasConcept C552990157 @default.
• W1971086498 hasConcept C78519656 @default.
• W1971086498 hasConcept C86803240 @default.
• W1971086498 hasConcept C92087593 @default.
• W1971086498 hasConceptScore W1971086498C104317684 @default.
• W1971086498 hasConceptScore W1971086498C107618985 @default.
• W1971086498 hasConceptScore W1971086498C127413603 @default.
• W1971086498 hasConceptScore W1971086498C130197536 @default.
• W1971086498 hasConceptScore W1971086498C13575015 @default.
• W1971086498 hasConceptScore W1971086498C141231307 @default.
• W1971086498 hasConceptScore W1971086498C1436000 @default.
• W1971086498 hasConceptScore W1971086498C153911025 @default.
• W1971086498 hasConceptScore W1971086498C168374021 @default.
• W1971086498 hasConceptScore W1971086498C182901222 @default.
• W1971086498 hasConceptScore W1971086498C187882448 @default.
• W1971086498 hasConceptScore W1971086498C19924922 @default.
• W1971086498 hasConceptScore W1971086498C2780098074 @default.
• W1971086498 hasConceptScore W1971086498C3017666073 @default.
• W1971086498 hasConceptScore W1971086498C40767141 @default.
• W1971086498 hasConceptScore W1971086498C42062724 @default.
• W1971086498 hasConceptScore W1971086498C523546767 @default.
• W1971086498 hasConceptScore W1971086498C54355233 @default.
• W1971086498 hasConceptScore W1971086498C552990157 @default.
• W1971086498 hasConceptScore W1971086498C78519656 @default.
• W1971086498 hasConceptScore W1971086498C86803240 @default.
• W1971086498 hasConceptScore W1971086498C92087593 @default.
• W1971086498 hasIssue "5" @default.
• W1971086498 hasLocation W19710864981 @default.
• W1971086498 hasLocation W19710864982 @default.
• W1971086498 hasOpenAccess W1971086498 @default.
• W1971086498 hasPrimaryLocation W19710864981 @default.

• next