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- W1971103637 abstract "Monoclonal antibodies have been produced to mammalian hair cell antigens using a simple in vitro kit. Antigen was crudely prepared from dissected cochlear tissue by detergent extraction. There was no need to purify hair cells. Hybridoma supernatents were screened most efficiently on dissociated cells fixed with acetone. The immunisation method is sensitive to nanograms of antigen and can generate responses to conserved or weak antigens. The kit requires very little previous experience with cell culture and generates monoclonal antibodies within 3–4 weeks. It has overcome a number of problems with production of antibodies to hair cells and it should prove to be a very valuable tool in many laboratories." @default.
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- W1971103637 date "1992-01-01" @default.
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- W1971103637 title "A simple in vitro method for raising monoclonal antibodies to cochlear proteins" @default.
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- W1971103637 doi "https://doi.org/10.1016/0040-8166(92)90032-3" @default.
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