Matches in SemOpenAlex for { <https://semopenalex.org/work/W1971172016> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1971172016 endingPage "34" @default.
- W1971172016 startingPage "26" @default.
- W1971172016 abstract "Abstract Human milk lipoprotein lipase (LPL) was purified by heparin-Sepharose 4B affinity chromatography. The time required for the purification was approximately 2 h. The acetone-diethyl ether powder of milk cream was extracted by a 0.1% Triton X-100 buffer solution and the extract was applied to the heparin-Sepharose 4B column. The partially purified LPL eluted by heparin had a specific activity of 5120 units/mg which represented a 2500-fold purification of the enzyme. The LPL was found to be stable in the heparin solution for at least 2 days at 4 °C. This enzyme preparation was found to be free of the bile salt-activated lipase activity, esterase activity, and cholesterol esterase activity. The LPL had no demonstrable basal activity with emulsified triolein in the absence of a serum cofactor. The enzyme was activated by serum and by apolipoprotein C-II. The application of milk LPL to studies on the in vitro degradation of human very low density lipoproteins can result in a 90–97% triglyceride hydrolysis. The LPL degraded very low density lipoprotein triglyceride and phospholipid without any effect on cholesterol esters. Of the partial glycerides potentially generated by lipolysis with milk LPL, only monoglycerides were present in measurable amounts after 60 min of lipolysis. These results show that the partially purified human milk LPL with its high specific activity and ease of purification represents a very suitable enzyme preparation for studying the kinetics and reaction mechanisms involved in the lipolytic degradation of human triglyceride-rich lipoproteins." @default.
- W1971172016 created "2016-06-24" @default.
- W1971172016 creator A5017604320 @default.
- W1971172016 creator A5036347143 @default.
- W1971172016 creator A5068537242 @default.
- W1971172016 creator A5075398812 @default.
- W1971172016 date "1982-03-01" @default.
- W1971172016 modified "2023-10-17" @default.
- W1971172016 title "Studies on the degradation of human very low density lipoproteins by human milk lipoprotein lipase" @default.
- W1971172016 cites W1498042732 @default.
- W1971172016 cites W1521846926 @default.
- W1971172016 cites W1555044531 @default.
- W1971172016 cites W1570360541 @default.
- W1971172016 cites W1608303427 @default.
- W1971172016 cites W1775749144 @default.
- W1971172016 cites W1880071198 @default.
- W1971172016 cites W1913984238 @default.
- W1971172016 cites W1945585777 @default.
- W1971172016 cites W1969954912 @default.
- W1971172016 cites W1987285410 @default.
- W1971172016 cites W1989744185 @default.
- W1971172016 cites W2003700385 @default.
- W1971172016 cites W2006103534 @default.
- W1971172016 cites W2006571152 @default.
- W1971172016 cites W2008351098 @default.
- W1971172016 cites W2027702065 @default.
- W1971172016 cites W2067708241 @default.
- W1971172016 cites W2082878166 @default.
- W1971172016 cites W2091649029 @default.
- W1971172016 cites W2118278614 @default.
- W1971172016 cites W2170352268 @default.
- W1971172016 cites W2184064314 @default.
- W1971172016 cites W2285946151 @default.
- W1971172016 cites W2329380505 @default.
- W1971172016 cites W2344308715 @default.
- W1971172016 cites W4300831047 @default.
- W1971172016 cites W65015235 @default.
- W1971172016 doi "https://doi.org/10.1016/0003-9861(82)90004-2" @default.
- W1971172016 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7082001" @default.
- W1971172016 hasPublicationYear "1982" @default.
- W1971172016 type Work @default.
- W1971172016 sameAs 1971172016 @default.
- W1971172016 citedByCount "34" @default.
- W1971172016 countsByYear W19711720162021 @default.
- W1971172016 crossrefType "journal-article" @default.
- W1971172016 hasAuthorship W1971172016A5017604320 @default.
- W1971172016 hasAuthorship W1971172016A5036347143 @default.
- W1971172016 hasAuthorship W1971172016A5068537242 @default.
- W1971172016 hasAuthorship W1971172016A5075398812 @default.
- W1971172016 hasConcept C181199279 @default.
- W1971172016 hasConcept C185592680 @default.
- W1971172016 hasConcept C2778163477 @default.
- W1971172016 hasConcept C2779679103 @default.
- W1971172016 hasConcept C2779697368 @default.
- W1971172016 hasConcept C2780072125 @default.
- W1971172016 hasConcept C31903555 @default.
- W1971172016 hasConcept C41008148 @default.
- W1971172016 hasConcept C55493867 @default.
- W1971172016 hasConcept C76155785 @default.
- W1971172016 hasConcept C94879076 @default.
- W1971172016 hasConceptScore W1971172016C181199279 @default.
- W1971172016 hasConceptScore W1971172016C185592680 @default.
- W1971172016 hasConceptScore W1971172016C2778163477 @default.
- W1971172016 hasConceptScore W1971172016C2779679103 @default.
- W1971172016 hasConceptScore W1971172016C2779697368 @default.
- W1971172016 hasConceptScore W1971172016C2780072125 @default.
- W1971172016 hasConceptScore W1971172016C31903555 @default.
- W1971172016 hasConceptScore W1971172016C41008148 @default.
- W1971172016 hasConceptScore W1971172016C55493867 @default.
- W1971172016 hasConceptScore W1971172016C76155785 @default.
- W1971172016 hasConceptScore W1971172016C94879076 @default.
- W1971172016 hasIssue "1" @default.
- W1971172016 hasLocation W19711720161 @default.
- W1971172016 hasLocation W19711720162 @default.
- W1971172016 hasOpenAccess W1971172016 @default.
- W1971172016 hasPrimaryLocation W19711720161 @default.
- W1971172016 hasRelatedWork W1552200847 @default.
- W1971172016 hasRelatedWork W1966312797 @default.
- W1971172016 hasRelatedWork W1988380490 @default.
- W1971172016 hasRelatedWork W1996595158 @default.
- W1971172016 hasRelatedWork W2013503996 @default.
- W1971172016 hasRelatedWork W2037447371 @default.
- W1971172016 hasRelatedWork W2070580253 @default.
- W1971172016 hasRelatedWork W2086855579 @default.
- W1971172016 hasRelatedWork W2414628146 @default.
- W1971172016 hasRelatedWork W3073573 @default.
- W1971172016 hasVolume "214" @default.
- W1971172016 isParatext "false" @default.
- W1971172016 isRetracted "false" @default.
- W1971172016 magId "1971172016" @default.
- W1971172016 workType "article" @default.