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• W1971210277 endingPage "1989" @default.
• W1971210277 startingPage "1973" @default.
• W1971210277 abstract "We have constructed new plasmids that can be used to clone transcription terminator containing DNA fragments between the first gene of the tryptophan ( trp ) operon and the tetracycline resistance ( tet ) gene. Both genes are under control of the trp promotor. Therefore the presence of a transcription termination signal on cloned fragments can be monitored by a decrease in expression of the tet gene. The plasmids contain cloning sites at different distances from the translation start signal. Consequently a cloned DNA fragment can be translated in the three possible reading frames, offering the opportunity to distinguish terminators from translation polarity (pseudo-terminators). The usefulness of the plasmids was shown by the cloning of the trp terminator and of a pseudo-terminator located in the trpB gene." @default.
• W1971210277 created "2016-06-24" @default.
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• W1971210277 date "1981-01-01" @default.
• W1971210277 modified "2023-10-15" @default.
• W1971210277 title "The construction of new vehicles for the cloning of transcription termination signals" @default.
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• W1971210277 doi "https://doi.org/10.1093/nar/9.8.1973" @default.
• W1971210277 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/326817" @default.
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