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• W1971230608 abstract "Base flipping, the conformational change of a nucleobase to an extrahelical position, is a key step in the enzymatic repair of damaged DNA. An assay that can detect the flipped-out species in free solution without covalent modification of the DNA would be desirable. The design and synthesis of a simple, sensitive, and rapid assay using specific noncovalent binding to pyrimidines by zinc-cyclen and a commonly used fluorescent reporter group, dansyl, is reported. The binding of the zinc-cyclen unit to a flipped-out thymine base results in a change in the fluorescent properties of the dansyl group that is distinct from nonspecific binding to duplex DNA or intercalation into either the flipped-in or flipped-out species. The assay was tested using fluorescence spectroscopy and detection at 533 +/- 5 nm with normal and abasic duplex DNA as negative and positive controls. The data obtained are fitted to a one-site binding model to determine the equilibrium constant for the two-step process involving base flipping and binding to be approximately 10-6 M." @default.
• W1971230608 created "2016-06-24" @default.
• W1971230608 creator A5029114040 @default.
• W1971230608 creator A5053585990 @default.
• W1971230608 date "2005-11-11" @default.
• W1971230608 modified "2023-10-17" @default.
• W1971230608 title "A Selective, Noncovalent Assay for Base Flipping in DNA" @default.
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• W1971230608 doi "https://doi.org/10.1021/ja056274+" @default.
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