FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1971338863> ?p ?o ?g. }

• W1971338863 endingPage "255" @default.
• W1971338863 startingPage "246" @default.
• W1971338863 abstract "UDP-galactose: N-acetylglucosamine galactosyltransferase (GT) and CMP-sialic:desialylated transferrin sialyltransferse (ST) activities of rat liver Golgi apparatus are membrane-bound enzymes that can be released by treatment with Triton X-100. When protein substrates are used to assay these enzymes in freshly prepared Golgi vesicles, both activities are enhanced about eightfold by the addition of Triton X-100. When small molecular weight substrates are used, however, both activities are only enhanced about twofold by the addition of detergent. The enzymes remain inaccessible to large protein substrates even after freezing and storage of the Golgi preparation for 2 mo in liquid nitrogen. Accessibility to small molecular and weight substrates increases significantly after such storage. GT and ST activities in Golgi vesicles are not destroyed by treatment with trypsin, but are destroyed by this treatment if the vesicles are first disrupted with Triton X-100. Treatment of Golgi vesicles with low levels of filipin, a polyene antibiotic known to complex with cholesterol in biological membranes, also results in enhanced trypsin susceptibility of both glycosyltransferases. Maximum destruction of the glycosyltransferase activities by trypsin is obtained at filipin to total cholesterol weight ratios of approximately 1.6 or molar ratios of approximately 1. This level of filipin does not solubilize the enzymes but causes both puckering of Golgi membranes visible by electron microscopy and disruption of the Golgi vesicles as measured by release of serum albumin. When isolated Golgi apparatus is fixed with glutaraldehyde to maintain the three-dimensional orientation of cisternae and secretory vesicles, and then treated with filipin, cisternal membranes on both cis and trans faces of the apparatus as well as secretory granule membranes appear to be affected about equally. These results indicate that liver Golgi vesicles as isolated are largely oriented with GT and ST on the luminal side of the membranes, which corresponds to the cisternal compartment of the Golgi apparatus in the hepatocyte. Cholesterol is an integral part of the membrane of the Golgi apparatus and its distribution throughout the apparatus is similar to that of both transferases." @default.
• W1971338863 created "2016-06-24" @default.
• W1971338863 creator A5087088035 @default.
• W1971338863 date "1981-05-01" @default.
• W1971338863 modified "2023-09-23" @default.
• W1971338863 title "Orientation of glycoprotein galactosyltransferase and sialyltransferase enzymes in vesicles derived from rat liver Golgi apparatus." @default.
• W1971338863 cites W1496493167 @default.
• W1971338863 cites W1501475854 @default.
• W1971338863 cites W1507779678 @default.
• W1971338863 cites W1537403674 @default.
• W1971338863 cites W1541125966 @default.
• W1971338863 cites W1542775374 @default.
• W1971338863 cites W1583024484 @default.
• W1971338863 cites W1599125820 @default.
• W1971338863 cites W1762028152 @default.
• W1971338863 cites W1775749144 @default.
• W1971338863 cites W1901274515 @default.
• W1971338863 cites W1983244318 @default.
• W1971338863 cites W1993078958 @default.
• W1971338863 cites W2000030956 @default.
• W1971338863 cites W2000167068 @default.
• W1971338863 cites W2003531044 @default.
• W1971338863 cites W2013892132 @default.
• W1971338863 cites W2031353470 @default.
• W1971338863 cites W2032627842 @default.
• W1971338863 cites W2035061398 @default.
• W1971338863 cites W2039485889 @default.
• W1971338863 cites W2043492836 @default.
• W1971338863 cites W2051779941 @default.
• W1971338863 cites W2052905130 @default.
• W1971338863 cites W2056750988 @default.
• W1971338863 cites W2056755246 @default.
• W1971338863 cites W2061787159 @default.
• W1971338863 cites W2072929329 @default.
• W1971338863 cites W2079185942 @default.
• W1971338863 cites W2080771304 @default.
• W1971338863 cites W2088627521 @default.
• W1971338863 cites W2088984996 @default.
• W1971338863 cites W2092437540 @default.
• W1971338863 cites W2092592958 @default.
• W1971338863 cites W2107265740 @default.
• W1971338863 cites W2140093238 @default.
• W1971338863 cites W2150729387 @default.
• W1971338863 cites W2166334672 @default.
• W1971338863 cites W2181039790 @default.
• W1971338863 cites W2206705755 @default.
• W1971338863 cites W2298223146 @default.
• W1971338863 cites W2401607841 @default.
• W1971338863 cites W2513210861 @default.
• W1971338863 cites W4229712605 @default.
• W1971338863 cites W71875362 @default.
• W1971338863 doi "https://doi.org/10.1083/jcb.89.2.246" @default.
• W1971338863 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2111678" @default.
• W1971338863 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6788776" @default.
• W1971338863 hasPublicationYear "1981" @default.
• W1971338863 type Work @default.
• W1971338863 sameAs 1971338863 @default.
• W1971338863 citedByCount "94" @default.
• W1971338863 countsByYear W19713388632021 @default.
• W1971338863 crossrefType "journal-article" @default.
• W1971338863 hasAuthorship W1971338863A5087088035 @default.
• W1971338863 hasBestOaLocation W19713388631 @default.
• W1971338863 hasConcept C119062480 @default.
• W1971338863 hasConcept C130316041 @default.
• W1971338863 hasConcept C158617107 @default.
• W1971338863 hasConcept C181199279 @default.
• W1971338863 hasConcept C2778322149 @default.
• W1971338863 hasConcept C2779630084 @default.
• W1971338863 hasConcept C2780115458 @default.
• W1971338863 hasConcept C2780340462 @default.
• W1971338863 hasConcept C2780895608 @default.
• W1971338863 hasConcept C2910033705 @default.
• W1971338863 hasConcept C41625074 @default.
• W1971338863 hasConcept C55493867 @default.
• W1971338863 hasConcept C86803240 @default.
• W1971338863 hasConceptScore W1971338863C119062480 @default.
• W1971338863 hasConceptScore W1971338863C130316041 @default.
• W1971338863 hasConceptScore W1971338863C158617107 @default.
• W1971338863 hasConceptScore W1971338863C181199279 @default.
• W1971338863 hasConceptScore W1971338863C2778322149 @default.
• W1971338863 hasConceptScore W1971338863C2779630084 @default.
• W1971338863 hasConceptScore W1971338863C2780115458 @default.
• W1971338863 hasConceptScore W1971338863C2780340462 @default.
• W1971338863 hasConceptScore W1971338863C2780895608 @default.
• W1971338863 hasConceptScore W1971338863C2910033705 @default.
• W1971338863 hasConceptScore W1971338863C41625074 @default.
• W1971338863 hasConceptScore W1971338863C55493867 @default.
• W1971338863 hasConceptScore W1971338863C86803240 @default.
• W1971338863 hasIssue "2" @default.
• W1971338863 hasLocation W19713388631 @default.
• W1971338863 hasLocation W19713388632 @default.
• W1971338863 hasLocation W19713388633 @default.
• W1971338863 hasLocation W19713388634 @default.
• W1971338863 hasOpenAccess W1971338863 @default.
• W1971338863 hasPrimaryLocation W19713388631 @default.
• W1971338863 hasRelatedWork W1971338863 @default.
• W1971338863 hasRelatedWork W2030658605 @default.
• W1971338863 hasRelatedWork W2039320662 @default.

• next