FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1971349746> ?p ?o ?g. }

• W1971349746 endingPage "1976" @default.
• W1971349746 startingPage "1967" @default.
• W1971349746 abstract "1. Previous studies have shown that the histamine H(1) receptor activates p42/p44 mitogen-activated protein kinases (MAPK) in DDT(1)MF-2 smooth muscle cells via a phosphatidylinositol 3-kinase (PI-3K)-dependent pathway. In this study the effect of histamine H(1) receptor stimulation on protein kinase B (PKB) and p70 S6 kinase, both of which are downstream targets of PI-3K, has been investigated. Increases in PKB and p70 S6 kinase activation were monitored by Western blotting using phospho-specific PKB (Ser(473)) and p70 S6 kinase (Thr(421)/Ser(424)) antibodies. 2. Histamine stimulated time and concentration-dependent increases in the phosphorylation of PKB and p70 S6 kinase in DDT(1)MF-2 cells. Both responses were completely inhibited by the histamine H(1) receptor antagonist mepyramine and following pre-treatment with pertussis toxin, to block G(i)/G(o) protein dependent pathways. 3. The PI-3K inhibitors wortmannin (IC(50) 5.9+/-0.5 nM) and LY 294002 (IC(50) 6.9+/-0.8 microM) attenuated the increase in PKB phosphorylation induced by histamine (100 microM) in a concentration-dependent manner. 4. Histamine-induced increases in p70 S6 kinase phosphorylation were partially sensitive to rapamycin (20 nM; 68% inhibition) but completely blocked by wortmannin (100 nM), LY 294002 (30 microM) and the MAPK kinase inhibitor PD 98059 (50 microM). 5. In summary, these data demonstrate that the histamine H(1) receptor stimulates PKB and p70 S6 kinase phosphorylation in DDT(1)MF-2 smooth muscle cells. However, functional studies revealed that histamine does not stimulate DDT(1)MF-2 cell proliferation or attenuate staurosporine-induced caspase-3 activity. The challenge for future research will be to link the stimulation of these kinase pathways with the physiological and pathophysiological roles of the histamine H(1) receptor." @default.
• W1971349746 created "2016-06-24" @default.
• W1971349746 creator A5034862572 @default.
• W1971349746 date "2002-04-01" @default.
• W1971349746 modified "2023-10-18" @default.
• W1971349746 title "Stimulation of protein kinase B and p70 S6 kinase by the histamine H₁receptor in DDT₁MF-2 smooth muscle cells" @default.
• W1971349746 cites W1529396499 @default.
• W1971349746 cites W1606738305 @default.
• W1971349746 cites W1629179716 @default.
• W1971349746 cites W1775749144 @default.
• W1971349746 cites W1791888878 @default.
• W1971349746 cites W1833083308 @default.
• W1971349746 cites W1911968642 @default.
• W1971349746 cites W1923904866 @default.
• W1971349746 cites W196003252 @default.
• W1971349746 cites W1963723155 @default.
• W1971349746 cites W1971915141 @default.
• W1971349746 cites W1983023635 @default.
• W1971349746 cites W1985460150 @default.
• W1971349746 cites W1998151245 @default.
• W1971349746 cites W2008679478 @default.
• W1971349746 cites W2009387204 @default.
• W1971349746 cites W2016085259 @default.
• W1971349746 cites W2020931266 @default.
• W1971349746 cites W2025182932 @default.
• W1971349746 cites W2030573603 @default.
• W1971349746 cites W2033379184 @default.
• W1971349746 cites W2035016947 @default.
• W1971349746 cites W2046867705 @default.
• W1971349746 cites W2049370911 @default.
• W1971349746 cites W2059946923 @default.
• W1971349746 cites W2061232760 @default.
• W1971349746 cites W2063117678 @default.
• W1971349746 cites W2064059449 @default.
• W1971349746 cites W2072780644 @default.
• W1971349746 cites W2078102222 @default.
• W1971349746 cites W2096549360 @default.
• W1971349746 cites W2097767970 @default.
• W1971349746 cites W2101741807 @default.
• W1971349746 cites W2103002807 @default.
• W1971349746 cites W2125542025 @default.
• W1971349746 cites W2129396889 @default.
• W1971349746 cites W2140994013 @default.
• W1971349746 cites W2149089744 @default.
• W1971349746 cites W2156174515 @default.
• W1971349746 cites W2156802607 @default.
• W1971349746 cites W2327972260 @default.
• W1971349746 cites W2336864235 @default.
• W1971349746 cites W2338971154 @default.
• W1971349746 cites W4242988300 @default.
• W1971349746 cites W48759033 @default.
• W1971349746 doi "https://doi.org/10.1038/sj.bjp.0704664" @default.
• W1971349746 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1573327" @default.
• W1971349746 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11959800" @default.
• W1971349746 hasPublicationYear "2002" @default.
• W1971349746 type Work @default.
• W1971349746 sameAs 1971349746 @default.
• W1971349746 citedByCount "21" @default.
• W1971349746 countsByYear W19713497462012 @default.
• W1971349746 countsByYear W19713497462015 @default.
• W1971349746 countsByYear W19713497462018 @default.
• W1971349746 countsByYear W19713497462021 @default.
• W1971349746 countsByYear W19713497462022 @default.
• W1971349746 crossrefType "journal-article" @default.
• W1971349746 hasAuthorship W1971349746A5034862572 @default.
• W1971349746 hasBestOaLocation W19713497462 @default.
• W1971349746 hasConcept C10335311 @default.
• W1971349746 hasConcept C104202773 @default.
• W1971349746 hasConcept C1122143 @default.
• W1971349746 hasConcept C11960822 @default.
• W1971349746 hasConcept C126322002 @default.
• W1971349746 hasConcept C134018914 @default.
• W1971349746 hasConcept C137361374 @default.
• W1971349746 hasConcept C153911025 @default.
• W1971349746 hasConcept C170493617 @default.
• W1971349746 hasConcept C184235292 @default.
• W1971349746 hasConcept C185592680 @default.
• W1971349746 hasConcept C2776885963 @default.
• W1971349746 hasConcept C2778308172 @default.
• W1971349746 hasConcept C55493867 @default.
• W1971349746 hasConcept C71924100 @default.
• W1971349746 hasConcept C75217442 @default.
• W1971349746 hasConcept C86803240 @default.
• W1971349746 hasConcept C90934575 @default.
• W1971349746 hasConcept C95444343 @default.
• W1971349746 hasConcept C97029542 @default.
• W1971349746 hasConceptScore W1971349746C10335311 @default.
• W1971349746 hasConceptScore W1971349746C104202773 @default.
• W1971349746 hasConceptScore W1971349746C1122143 @default.
• W1971349746 hasConceptScore W1971349746C11960822 @default.
• W1971349746 hasConceptScore W1971349746C126322002 @default.
• W1971349746 hasConceptScore W1971349746C134018914 @default.
• W1971349746 hasConceptScore W1971349746C137361374 @default.
• W1971349746 hasConceptScore W1971349746C153911025 @default.
• W1971349746 hasConceptScore W1971349746C170493617 @default.
• W1971349746 hasConceptScore W1971349746C184235292 @default.
• W1971349746 hasConceptScore W1971349746C185592680 @default.
• W1971349746 hasConceptScore W1971349746C2776885963 @default.

• next