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- W1971455858 abstract "When propagated in cesium-rich medium, type 1 poliovirus incorporated enough cesium atoms to shift its buoyant density from 1.34 to an upper limit of about 1.40 g/ml. Studies with radioactive 137Cs indicated that this upper density limit corresponded to about 4200 cesium atoms per virion. The incorporated cesium was tightly bound, exchanging out of virions at a negligible rate at temperatures below 22°C. Cesium-loaded virions were normal with respect to specific infectivity, neutralizability by specific antisera, and electrophoretic profile of coat protein. Natural top component bound fewer than 20 cesium atoms per particle under the same conditions. These results demonstrate that the protein coat of poliovirus is impermeable to cesium ions. However, if the RNA of the virus is exposed to cesium ions while the virus is being assembled, the poliovirus binds approximately the same number of cesium ions as human rhinovirus. This similarity in cesium binding suggests that poliovirus and human rhinovirus may have the same fraction of their RNA neutralized by coat protein." @default.
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- W1971455858 date "1978-10-01" @default.
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- W1971455858 title "Properties of poliovirus propagated in medium containing cesium chloride: Implications for picornaviral structure" @default.
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- W1971455858 doi "https://doi.org/10.1016/0042-6822(78)90337-9" @default.
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