Matches in SemOpenAlex for { <https://semopenalex.org/work/W1971460289> ?p ?o ?g. }
- W1971460289 endingPage "4936" @default.
- W1971460289 startingPage "4925" @default.
- W1971460289 abstract "Extracellular matrix (ECM) production is critical to preserve the function and integrity of mature blood vessels. Toward the engineering of blood vessels, studies have centered on ECM production by supporting cells, whereas few studies implicate endothelial cells (ECs) with ECM synthesis. Here, we elucidate variations between cultured human arterial, venous, and progenitor ECs with respect to ECM deposition assembly, composition, and response to biomolecular and physiological factors. Our studies reveal that progenitor ECs, endothelial colony-forming cells (ECFCs), deposit collagen IV, fibronectin, and laminin that assemble to an organized weblike structure, as confirmed by decellularized cultures. Mature ECs only express these ECM proteins intracellularly. ECFC-derived ECM is abrogated in response to TGFβ signaling inhibition and actin cytoskeleton disruption. Hypoxic (1%) and physiological (5%) O(2) tension stimulate ECM deposition from mature ECs. Interestingly, deposition of collagen I is observed only under 5% O(2) tension. ECM production from all ECs is found to be regulated by hypoxia-inducible factors 1α and 2α but differentially in the different cell lines. Collectively, we suggest that ECM deposition and assembly by ECs is dependent on maturation stage and oxygen supply and that these findings can be harnessed to advance engineered vascular therapeutics." @default.
- W1971460289 created "2016-06-24" @default.
- W1971460289 creator A5009744120 @default.
- W1971460289 creator A5019329595 @default.
- W1971460289 creator A5032580711 @default.
- W1971460289 date "2012-08-23" @default.
- W1971460289 modified "2023-10-16" @default.
- W1971460289 title "The extracellular matrix is a novel attribute of endothelial progenitors and of hypoxic mature endothelial cells" @default.
- W1971460289 cites W1569444222 @default.
- W1971460289 cites W1586474677 @default.
- W1971460289 cites W1965182712 @default.
- W1971460289 cites W1970564951 @default.
- W1971460289 cites W1973913381 @default.
- W1971460289 cites W1975863665 @default.
- W1971460289 cites W1976180996 @default.
- W1971460289 cites W1976980661 @default.
- W1971460289 cites W1979103623 @default.
- W1971460289 cites W1984809732 @default.
- W1971460289 cites W1986991023 @default.
- W1971460289 cites W1995629728 @default.
- W1971460289 cites W1996575975 @default.
- W1971460289 cites W2001991657 @default.
- W1971460289 cites W2002649920 @default.
- W1971460289 cites W2008062232 @default.
- W1971460289 cites W2010386340 @default.
- W1971460289 cites W2012709017 @default.
- W1971460289 cites W2013657120 @default.
- W1971460289 cites W2018008610 @default.
- W1971460289 cites W2019759729 @default.
- W1971460289 cites W2021585018 @default.
- W1971460289 cites W2022451432 @default.
- W1971460289 cites W2023148812 @default.
- W1971460289 cites W2025341270 @default.
- W1971460289 cites W2027398512 @default.
- W1971460289 cites W2030495747 @default.
- W1971460289 cites W2031856001 @default.
- W1971460289 cites W2035595755 @default.
- W1971460289 cites W2039787319 @default.
- W1971460289 cites W2041446130 @default.
- W1971460289 cites W2047470479 @default.
- W1971460289 cites W2047749203 @default.
- W1971460289 cites W2065864290 @default.
- W1971460289 cites W2067952294 @default.
- W1971460289 cites W2069800121 @default.
- W1971460289 cites W2072434050 @default.
- W1971460289 cites W2076224797 @default.
- W1971460289 cites W2077844244 @default.
- W1971460289 cites W2085686791 @default.
- W1971460289 cites W2086307231 @default.
- W1971460289 cites W2090409777 @default.
- W1971460289 cites W2094899281 @default.
- W1971460289 cites W2101228997 @default.
- W1971460289 cites W2101480539 @default.
- W1971460289 cites W2105683352 @default.
- W1971460289 cites W2111338636 @default.
- W1971460289 cites W2115107127 @default.
- W1971460289 cites W2118877693 @default.
- W1971460289 cites W2120439608 @default.
- W1971460289 cites W2121348589 @default.
- W1971460289 cites W2122388238 @default.
- W1971460289 cites W2133476128 @default.
- W1971460289 cites W2134272331 @default.
- W1971460289 cites W2138179190 @default.
- W1971460289 cites W2140618470 @default.
- W1971460289 cites W2141738503 @default.
- W1971460289 cites W2143156113 @default.
- W1971460289 cites W2143734834 @default.
- W1971460289 cites W2152442991 @default.
- W1971460289 cites W2154558435 @default.
- W1971460289 cites W2155958464 @default.
- W1971460289 cites W2163075033 @default.
- W1971460289 cites W2168125520 @default.
- W1971460289 cites W2169608796 @default.
- W1971460289 cites W2171006416 @default.
- W1971460289 cites W2171173782 @default.
- W1971460289 cites W2171828133 @default.
- W1971460289 cites W2172264076 @default.
- W1971460289 cites W2240765188 @default.
- W1971460289 doi "https://doi.org/10.1096/fj.12-209296" @default.
- W1971460289 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3509053" @default.
- W1971460289 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22919069" @default.
- W1971460289 hasPublicationYear "2012" @default.
- W1971460289 type Work @default.
- W1971460289 sameAs 1971460289 @default.
- W1971460289 citedByCount "63" @default.
- W1971460289 countsByYear W19714602892013 @default.
- W1971460289 countsByYear W19714602892014 @default.
- W1971460289 countsByYear W19714602892015 @default.
- W1971460289 countsByYear W19714602892016 @default.
- W1971460289 countsByYear W19714602892017 @default.
- W1971460289 countsByYear W19714602892018 @default.
- W1971460289 countsByYear W19714602892019 @default.
- W1971460289 countsByYear W19714602892020 @default.
- W1971460289 countsByYear W19714602892021 @default.
- W1971460289 countsByYear W19714602892022 @default.
- W1971460289 countsByYear W19714602892023 @default.
- W1971460289 crossrefType "journal-article" @default.
- W1971460289 hasAuthorship W1971460289A5009744120 @default.