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- W1971513828 abstract "Because of the growing importance of the Retinoid X Receptors (RXRα, β and γ) in the retinoid acid signalling pathway, we have prepared polyclonal and monoclonal antibodies directed against these proteins. For this purpose, either the whole mouse RXRα protein expressed in E.Coli, or synthetic peptides corresponding to amino acid sequences common to all RXRs or unique to RXRα, β or γ, were used as antigens. Antibodies recognizing either all three RXR types (α, β and γ) or specific for each RXR type were obtained. The antibodies were characterized by immunocytochemistry, immunoblotting, immunoprecipitation and electromobility shift assay (EMSA). These antibodies allowed us to detect the presence of RXRα proteins in mouse embryos and in mouse embryonal carcinoma cells (F9 and P19 cell lines) by immunoblotting, immunoprecipitation and EMSA whereas RXRβ could be detected only by EMSA and RXRγ could not be detected by any of these techniques." @default.
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- W1971513828 date "1994-10-01" @default.
- W1971513828 modified "2023-10-18" @default.
- W1971513828 title "Detection of Retinoid X Receptors Using Specific Monoclonal and Polyclonal Antibodies" @default.
- W1971513828 doi "https://doi.org/10.1006/bbrc.1994.2491" @default.
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