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- W1971538795 abstract "A simple, rapid, mechanical procedure for the isolation of intact mesophyll cells within 2–3 min from soybean leaves is described. The identification of isolated mesophyll cells was made by light microscopy. Contamination of the preparation by chloroplasts was minimal. The intactness of the plasmalemma was checked by Evan's blue impermeability, phase contrast microscopy, the ability to be conversted to protoplasts and the specific exclusion of lipophobic protein modifiers. Maximum rates of photosynthesis by these cells occured at 35°C, pH 7.8, 4 mM HCO3, and 800–1200 μE/m2/s (400–700 nM). A pre-illumination period of 5 min was essential for maximum rates of photosynthesis. Photosynthesis rates with the isolated mesophyll cells averaged 150 μmol CO2/mg chlorophyll/h for preparations made over a period of several months and occasionally approached 300μmol/mg chlorophyll/h." @default.
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- W1971538795 date "1979-10-01" @default.
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- W1971538795 title "Rapid isolation of mesophyll cells from soybean leaves" @default.
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- W1971538795 doi "https://doi.org/10.1016/0304-4211(79)90022-1" @default.
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