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• W1971585008 startingPage "332" @default.
• W1971585008 abstract "5('),5(')-Adenylyl pyrophosphoryl DNA (AppDNA) contains a high-energy pyrophosphate linkage and can be exploited as an activated DNA substrate to derive new DNA enzymes for carrying out various DNA modification reactions. For this reason, enzymatic synthesis of AppDNA is highly desirable. AppDNA is a known intermediate in DNA ligase mediated DNA ligation reactions, but rarely accumulates under normal reaction conditions. Here we report that T4 DNA ligase can quantitatively convert 5(')-phosphoryl DNA donor into AppDNA in the absence of acceptor DNA but in the presence of a template DNA that contains at least one unpaired nucleotide opposite the 5(')-phosphoryl DNA donor site. This adenylylation behavior of T4 DNA ligase is not observed with Thermus aquaticus (Taq) and Escherichia coli DNA ligases. We further found that a donor-template duplex of 11-bp in length is required by T4 DNA ligase for the formation of AppDNA." @default.
• W1971585008 created "2016-06-24" @default.
• W1971585008 creator A5039579810 @default.
• W1971585008 creator A5081367802 @default.
• W1971585008 date "2002-10-01" @default.
• W1971585008 modified "2023-10-12" @default.
• W1971585008 title "Making AppDNA using T4 DNA ligase" @default.
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• W1971585008 doi "https://doi.org/10.1016/s0045-2068(02)00018-4" @default.
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