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• W1971585483 abstract "The electron density of cytoplasmic granules, 50–100 nm in diameter, observed in unstained thin sections of log-phase Nocardia corallina varied with the fixation procedure. In organisms fixed with glutaraldehyde alone the granules were electron-translucent, while in organisms fixed with glutaraldehyde followed by osmium tetroxide the granules were electron-dense. When postfixation in uranyl acetate followed glutaraldehyde-osmium tetroxide fixation, the granules appeared less electron-dense than those prepared with comparable fixatives in which uranyl acetate was absent. The granules stained positively with the Thiery silver stain for polysaccharides with all methods of fixation. Cell walls, cytoplasmic membranes, and mesosomes also stained positively with the Thiery stain. Treatments omitting various reagents in the Thiery method or including hydrogen peroxide or the aldehyde blocking agent, sodium borohydride, confirmed the specificity of the stain and also demonstrated that differences in electron density of the granules in unstained thin sections were related to the amount of bound osmium and not to extraction of the granules." @default.
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• W1971585483 date "1975-09-01" @default.
• W1971585483 modified "2023-09-26" @default.
• W1971585483 title "The effect of fixation procedures on the electron density of polysaccharide granules in Nocardia coralina" @default.
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• W1971585483 doi "https://doi.org/10.1016/s0022-5320(75)80072-4" @default.
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