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- W1971633233 abstract "1. A method for the determination of lipoprotein lipase activity in small samples of human adipose tissue obtained by aspiration biopsy is reported. After extraction with acetone and ether, the sample is incubated with a fatty acid-labeled triacylglycerol emulsion activated with serum, and released fatty acids directly isolated in a liquid-liquid partition system. A specially designed microhomogenizer-incubation vessel allows the entire procedure to be performed in a single vial. 2. Lipoprotein lipase activity of the biopsy specimen was obtained with high recovery in the acetone-ether preparation, which also contained an excess of monoglyceride esterase activity. Essentially none of the latter was measured under the conditions of the assay. The measured activity was almost completely dependent on the presence of serum, strongly inhibited by 1 M Nad and protamine sulfate, had a pH optimum of 8.0, and rapidly increased on carbohydrate feeding, substantiating the high specificity for lipoprotein lipase. 3. Ten to twenty mg of human adipose tissue were sufficient for the determination of lipoprotein lipase activity, allowing serial sampling with small inconvenience to the subject. The entire procedure requires less than 3 h and 20 determinations can be performed in 4 h." @default.
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- W1971633233 date "1972-12-01" @default.
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- W1971633233 title "Rapid determination of lipoprotein lipase activity in human adipose tissue" @default.
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- W1971633233 doi "https://doi.org/10.1016/0009-8981(72)90103-9" @default.
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