Matches in SemOpenAlex for { <https://semopenalex.org/work/W1971636127> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1971636127 endingPage "114" @default.
- W1971636127 startingPage "107" @default.
- W1971636127 abstract "Growth rates of different clones, all derived from NIH 3T3 mouse fibroblasts, were determined. Four different types of cells were studied: (1) Normal NIH 3T3 fibroblasts; (2) a fast-growing NIH 3T3 clone obtained by repeated passages; (3) transformed clones (obtained by transfecting NIH 3T3 with the oncogene c-Ha-ras); (4) a slow-growing revertant obtained by repeated passages of the transformed line. Growth rates were determined by the following markers of proliferation: thymidine incorporation, protein accumulation and cell number. In parallel experiments growth rates were determined by a new approach based on measuring ornithine decarboxylase (ODC) activity. Transformed cells, which were characterized by phase-contrast microscopy and by electron microscopy grew rapidly and showed high ODC activity. Similarly, a high-passage NIH 3T3 variant, which grew rapidly, also possessed high ODC activity. On the other hand, high-passage of a transformed clone revealed phenotypic changes confirmed by electron microscopy. These cells exhibited reduced growth rates and their ODC activities were similar to those of the normal NIH 3T3 cells. A confident correlation was found among each of the three conventional parameters of growth and between them and ODC activity. However, in all the cases studied ODC activity appeared early in the cell cycle before the expression of the other markers of proliferation. It has been suggested that ODC is a reliable early marker of cell proliferation and might also serve as an important tool for determining the arrest of growth." @default.
- W1971636127 created "2016-06-24" @default.
- W1971636127 creator A5008597633 @default.
- W1971636127 creator A5076068942 @default.
- W1971636127 date "1995-06-01" @default.
- W1971636127 modified "2023-09-27" @default.
- W1971636127 title "Ornithine decarboxylase: An indicator for growth of NIH 3T3 fibroblasts and their c-Ha-ras transformants" @default.
- W1971636127 cites W1616429637 @default.
- W1971636127 cites W1965372133 @default.
- W1971636127 cites W1965951291 @default.
- W1971636127 cites W1980334365 @default.
- W1971636127 cites W1988843247 @default.
- W1971636127 cites W2009950824 @default.
- W1971636127 cites W2013973825 @default.
- W1971636127 cites W2015234889 @default.
- W1971636127 cites W2024573207 @default.
- W1971636127 cites W2030879518 @default.
- W1971636127 cites W2031468048 @default.
- W1971636127 cites W2043093940 @default.
- W1971636127 cites W2043985253 @default.
- W1971636127 cites W2072871825 @default.
- W1971636127 cites W2080383188 @default.
- W1971636127 cites W2082634075 @default.
- W1971636127 cites W2100837269 @default.
- W1971636127 cites W2113119645 @default.
- W1971636127 cites W2237344699 @default.
- W1971636127 cites W4238270754 @default.
- W1971636127 cites W4293247451 @default.
- W1971636127 doi "https://doi.org/10.1016/0167-4889(95)00039-u" @default.
- W1971636127 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7612662" @default.
- W1971636127 hasPublicationYear "1995" @default.
- W1971636127 type Work @default.
- W1971636127 sameAs 1971636127 @default.
- W1971636127 citedByCount "14" @default.
- W1971636127 countsByYear W19716361272012 @default.
- W1971636127 countsByYear W19716361272016 @default.
- W1971636127 crossrefType "journal-article" @default.
- W1971636127 hasAuthorship W1971636127A5008597633 @default.
- W1971636127 hasAuthorship W1971636127A5076068942 @default.
- W1971636127 hasConcept C121289238 @default.
- W1971636127 hasConcept C1491633281 @default.
- W1971636127 hasConcept C153911025 @default.
- W1971636127 hasConcept C181199279 @default.
- W1971636127 hasConcept C2776746612 @default.
- W1971636127 hasConcept C2780381497 @default.
- W1971636127 hasConcept C29537977 @default.
- W1971636127 hasConcept C54009773 @default.
- W1971636127 hasConcept C54355233 @default.
- W1971636127 hasConcept C552990157 @default.
- W1971636127 hasConcept C55493867 @default.
- W1971636127 hasConcept C62112901 @default.
- W1971636127 hasConcept C81089528 @default.
- W1971636127 hasConcept C81885089 @default.
- W1971636127 hasConcept C85813293 @default.
- W1971636127 hasConcept C86803240 @default.
- W1971636127 hasConceptScore W1971636127C121289238 @default.
- W1971636127 hasConceptScore W1971636127C1491633281 @default.
- W1971636127 hasConceptScore W1971636127C153911025 @default.
- W1971636127 hasConceptScore W1971636127C181199279 @default.
- W1971636127 hasConceptScore W1971636127C2776746612 @default.
- W1971636127 hasConceptScore W1971636127C2780381497 @default.
- W1971636127 hasConceptScore W1971636127C29537977 @default.
- W1971636127 hasConceptScore W1971636127C54009773 @default.
- W1971636127 hasConceptScore W1971636127C54355233 @default.
- W1971636127 hasConceptScore W1971636127C552990157 @default.
- W1971636127 hasConceptScore W1971636127C55493867 @default.
- W1971636127 hasConceptScore W1971636127C62112901 @default.
- W1971636127 hasConceptScore W1971636127C81089528 @default.
- W1971636127 hasConceptScore W1971636127C81885089 @default.
- W1971636127 hasConceptScore W1971636127C85813293 @default.
- W1971636127 hasConceptScore W1971636127C86803240 @default.
- W1971636127 hasIssue "2-3" @default.
- W1971636127 hasLocation W19716361271 @default.
- W1971636127 hasLocation W19716361272 @default.
- W1971636127 hasOpenAccess W1971636127 @default.
- W1971636127 hasPrimaryLocation W19716361271 @default.
- W1971636127 hasRelatedWork W2033555049 @default.
- W1971636127 hasRelatedWork W2066519212 @default.
- W1971636127 hasRelatedWork W2072481810 @default.
- W1971636127 hasRelatedWork W209272479 @default.
- W1971636127 hasRelatedWork W2361326612 @default.
- W1971636127 hasRelatedWork W2363880822 @default.
- W1971636127 hasRelatedWork W2388699538 @default.
- W1971636127 hasRelatedWork W2397559227 @default.
- W1971636127 hasRelatedWork W315696163 @default.
- W1971636127 hasRelatedWork W4291127362 @default.
- W1971636127 hasVolume "1267" @default.
- W1971636127 isParatext "false" @default.
- W1971636127 isRetracted "false" @default.
- W1971636127 magId "1971636127" @default.
- W1971636127 workType "article" @default.