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- W1971673994 abstract "Previously we established that in vitro NO2 exposure induced inhibition of histamine release from rat peritoneal mast cells (PMC) stimulated with secretagogues such as compound 48/80 or substance P. To further explore the effects of NO2 exposure on mast cells, we investigated whether the addition of an antioxidant agent, 2-mercaptoethanol (2-ME), can prevent NO2-induced inhibition of mediator release from PMC. Histamine release from 5 ppm NO2-exposed PMC stimulated with 10 and 20 μm substance P was significantly inhibited compared with that from the controls. β-Hexosaminidase release from 5 ppm NO2-exposed PMC stimulated with 20 μm substance P was also significantly inhibited. However, the inhibition of both histamine and β-hexosaminidase release from exposed PMC was diminished by the addition of 5 mm 2-ME during NO2 exposure. Although IgE-mediated histamine release from NO2 exposed PMC was markedly inhibited, the addition of 5 mm 2-ME during NO2 exposure induced no inhibition of histamine release. These results suggest a possible relationship between NO2-induced inhibition of mast cell mediator release and production of free radicals by the action of NO2." @default.
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- W1971673994 date "1990-12-01" @default.
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- W1971673994 title "An antioxidant agent prevents NO2-induced inhibition of mast cell mediator release: Evidence that the mechanism involves free radicals" @default.
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- W1971673994 doi "https://doi.org/10.1016/s0013-9351(05)80117-3" @default.
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