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- W1971774923 abstract "Regular case work on sexual assaults, with one or multiple perpetrators, is challenging as such cases have many different aspects to take into account. Often, the limitations is due to the time frame between the alleged assault and the sample collection, the victim's activities in this time period, samples or clothing collected and the lack of reference samples from the victim, sexual partners, or the alleged perpetrator(s). Literature has shown that there are a correlation between the acid phosphatase reaction/seminal fluid or detection of spermatozoa and the time frame between deposition and sample collection [1Allard J.E. et al.The collection of data from findings in cases of sexual assault and the significance of spermatozoa on vaginal, anal and oral swabs.Sci. Justice. 1997; 37: 99-108Abstract Full Text PDF PubMed Scopus (50) Google Scholar, 2Culhane J.F. et al.Evaluation of semen detection in vaginal secretions: comparison of four methods.Am. J. Reprod. Immunol. 2008; 60: 274-281Crossref PubMed Scopus (14) Google Scholar, 3Willott G.M. Allard J.E. Spermatozoa – their persistence after sexual intercourse.Forensic Sci. Int. 1982; 19: 135-154Abstract Full Text PDF PubMed Scopus (86) Google Scholar, 4Hellerud B.B. et al.Sperm detection: a retrospective overview from 2010.Forensic Sci. Int.: Genet. Suppl. Ser. 2011; 3: e391-e392Abstract Full Text Full Text PDF Scopus (8) Google Scholar]. However, we do not know whether the seminal fluid and amount of spermatozoa detected are evenly distributed throughout a stain, and if or how the sampling affects the DNA-results, especially in complex mixtures.In this sexual assault case, from 2011, the exhibits and body samples were first routinely investigated. The presumptive test (acid phosphatase Brentamine) produced positive reactions on all exhibits. Numerous spermatozoa were observed by microscopy in all collected samples. All fractions were analyzed using AmpFlSTR® SGM Plus kit (Applied Biosystems). Further, both ESX 17® kit (Promega) and Yfiler® kit (Applied Biosystems) were used for a selection of the sperm fractions.The results of all the analyzed sperm fractions showed complex DNA mixtures, with at least three and/or four contributors, in different mixture ratios. By using the different mixture proportions and deconvolution the DNA-profiles of two contributors could be deduced for all markers. A database search produced an “offender hit” for one of these profiles. As a final remark, the results in this case show that the sampling can be essential, and that the distribution of spermatozoa is not evenly distributed throughout a stain. The results indicate that it may be recommended to select several samples from the stain(s) in cases with multiple perpetrators and complex DNA-results." @default.
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- W1971774923 date "2013-01-01" @default.
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- W1971774923 title "A rape case with multiple unknown perpetrators" @default.
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- W1971774923 doi "https://doi.org/10.1016/j.fsigss.2013.10.184" @default.
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