FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1971834947> ?p ?o ?g. }

• W1971834947 endingPage "313" @default.
• W1971834947 startingPage "307" @default.
• W1971834947 abstract "Gene delivery using cationic polymers such as chitosan shows good biocompatibility, but reveals low transfection efficiency. Fibronectin Attachment Protein of Mycobacterium bovis (FAP-B) which is responsible for the attachment of many Mycobacteria on the Fibronectin molecule of epithelial cell membrane can be considered as a new targeting ligand and can improve transfection rates in epithelial cells. In this study, chitosan–DNA nanoparticles were prepared using coacervation process. The effect of stirring speed and charge ratio (N/P) on the size and zeta potential of nanoparticles were evaluated. FAP-B ligand was added to nanoparticles at the specific condition to form chitosan–DNA–FAP-B nanoparticles via electrostatic attraction. Transfection efficiency of the final nanoparticles was investigated in A549 (alveolar epithelial cells). Cell viability was investigated using MTT assay. The optimum speed of stirring which was yielded the smallest chitosan–DNA nanoparticles with a narrow distribution (227 ± 43 nm), was 500 rpm with the corresponding N/P ratio of 20. Chitosan–DNA–FAP-B nanoparticles presented the size of 279 ± 27 nm with transfection efficiency about 10-fold higher than chitosan–DNA nanoparticles and resulted in 97.3% cell viability compared to 71.7% using Turbofect controls. Chitosan–DNA–FAP-B nanoparticles showed good transfection efficiency without cell toxicity. They have small particle size around 279 nm which make them a promising candidate as a novel non-viral gene vector for gene delivery to lung epithelial cells." @default.
• W1971834947 created "2016-06-24" @default.
• W1971834947 creator A5002216274 @default.
• W1971834947 creator A5004219128 @default.
• W1971834947 creator A5022450548 @default.
• W1971834947 creator A5023989350 @default.
• W1971834947 creator A5043440312 @default.
• W1971834947 creator A5052150029 @default.
• W1971834947 creator A5080812882 @default.
• W1971834947 creator A5088557823 @default.
• W1971834947 date "2011-05-01" @default.
• W1971834947 modified "2023-10-16" @default.
• W1971834947 title "Preparation and evaluation of chitosan–DNA–FAP-B nanoparticles as a novel non-viral vector for gene delivery to the lung epithelial cells" @default.
• W1971834947 cites W1511118087 @default.
• W1971834947 cites W1560355462 @default.
• W1971834947 cites W1835120563 @default.
• W1971834947 cites W1969899416 @default.
• W1971834947 cites W1978643345 @default.
• W1971834947 cites W1987905566 @default.
• W1971834947 cites W1992909684 @default.
• W1971834947 cites W2006721103 @default.
• W1971834947 cites W2009307175 @default.
• W1971834947 cites W2010716123 @default.
• W1971834947 cites W2013523297 @default.
• W1971834947 cites W2024907303 @default.
• W1971834947 cites W2026013892 @default.
• W1971834947 cites W2038984754 @default.
• W1971834947 cites W2041426309 @default.
• W1971834947 cites W2041874034 @default.
• W1971834947 cites W2056932561 @default.
• W1971834947 cites W2057456161 @default.
• W1971834947 cites W2062820647 @default.
• W1971834947 cites W2068917179 @default.
• W1971834947 cites W2072430760 @default.
• W1971834947 cites W2073550930 @default.
• W1971834947 cites W2082641947 @default.
• W1971834947 cites W2082721939 @default.
• W1971834947 cites W2083960630 @default.
• W1971834947 cites W2086586009 @default.
• W1971834947 cites W2095507086 @default.
• W1971834947 cites W2095757303 @default.
• W1971834947 cites W2101532425 @default.
• W1971834947 cites W2102616280 @default.
• W1971834947 cites W2126305627 @default.
• W1971834947 cites W2316709739 @default.
• W1971834947 cites W4238711183 @default.
• W1971834947 doi "https://doi.org/10.1016/j.ijpharm.2011.02.043" @default.
• W1971834947 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21356293" @default.
• W1971834947 hasPublicationYear "2011" @default.
• W1971834947 type Work @default.
• W1971834947 sameAs 1971834947 @default.
• W1971834947 citedByCount "39" @default.
• W1971834947 countsByYear W19718349472012 @default.
• W1971834947 countsByYear W19718349472013 @default.
• W1971834947 countsByYear W19718349472014 @default.
• W1971834947 countsByYear W19718349472015 @default.
• W1971834947 countsByYear W19718349472016 @default.
• W1971834947 countsByYear W19718349472017 @default.
• W1971834947 countsByYear W19718349472018 @default.
• W1971834947 countsByYear W19718349472019 @default.
• W1971834947 countsByYear W19718349472020 @default.
• W1971834947 countsByYear W19718349472021 @default.
• W1971834947 countsByYear W19718349472023 @default.
• W1971834947 crossrefType "journal-article" @default.
• W1971834947 hasAuthorship W1971834947A5002216274 @default.
• W1971834947 hasAuthorship W1971834947A5004219128 @default.
• W1971834947 hasAuthorship W1971834947A5022450548 @default.
• W1971834947 hasAuthorship W1971834947A5023989350 @default.
• W1971834947 hasAuthorship W1971834947A5043440312 @default.
• W1971834947 hasAuthorship W1971834947A5052150029 @default.
• W1971834947 hasAuthorship W1971834947A5080812882 @default.
• W1971834947 hasAuthorship W1971834947A5088557823 @default.
• W1971834947 hasConcept C104317684 @default.
• W1971834947 hasConcept C12554922 @default.
• W1971834947 hasConcept C135983454 @default.
• W1971834947 hasConcept C1491633281 @default.
• W1971834947 hasConcept C153911025 @default.
• W1971834947 hasConcept C155672457 @default.
• W1971834947 hasConcept C171250308 @default.
• W1971834947 hasConcept C185592680 @default.
• W1971834947 hasConcept C192562407 @default.
• W1971834947 hasConcept C2779732960 @default.
• W1971834947 hasConcept C53227056 @default.
• W1971834947 hasConcept C54009773 @default.
• W1971834947 hasConcept C55493867 @default.
• W1971834947 hasConcept C83029808 @default.
• W1971834947 hasConcept C86181022 @default.
• W1971834947 hasConcept C86803240 @default.
• W1971834947 hasConceptScore W1971834947C104317684 @default.
• W1971834947 hasConceptScore W1971834947C12554922 @default.
• W1971834947 hasConceptScore W1971834947C135983454 @default.
• W1971834947 hasConceptScore W1971834947C1491633281 @default.
• W1971834947 hasConceptScore W1971834947C153911025 @default.
• W1971834947 hasConceptScore W1971834947C155672457 @default.
• W1971834947 hasConceptScore W1971834947C171250308 @default.
• W1971834947 hasConceptScore W1971834947C185592680 @default.
• W1971834947 hasConceptScore W1971834947C192562407 @default.
• W1971834947 hasConceptScore W1971834947C2779732960 @default.

• next