FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1971859563> ?p ?o ?g. }

• W1971859563 endingPage "515" @default.
• W1971859563 startingPage "506" @default.
• W1971859563 abstract "Rationale: Sildenafil has been implicated in the activation of cystic fibrosis transmembrane conductance regulator (CFTR) protein. The effect was observed in vitro and in the presence of doses roughly 300 times larger than those commonly used for treating erectile dysfunction.Objectives: To evaluate in vivo the therapeutic efficacy of clinical doses of sildenafil and vardenafil, two clinically approved phosphodiesterase 5 inhibitors, for activating ion transport in cystic fibrosis.Methods: We used transepithelial potential difference in vivo across the nasal mucosa as a measure of sodium and chloride transport. The effect of a single intraperitoneal injection of sildenafil (0.7 mg/kg) or vardenafil (0.14 mg/kg) was investigated in F508del, cftr knockout and normal homozygous mice.Measurements and Main Results: In F508del mice, but not in cftr knockout mice, the chloride conductance, evaluated by perfusing the nasal mucosa with a chloride-free solution in the presence of amiloride and with forskolin, was corrected 1 hour after sildenafil administration. A more prolonged effect, persisting for at least 24 hours, was observed with vardenafil. The forskolin response was increased after sildenafil and vardenafil in both normal and F508del mutant animals. In F508del mice, the chloride conductance in the presence of 200 μM 4-4′-diisothiocyanostilbene-2,2′-disulphonic acid, an inhibitor of alternative chloride channels, was much higher after sildenafil injection than after placebo treatment. No effect on the sodium conductance was detected in any group of animals.Conclusions: Our results provide preclinical evidence that both drugs stimulate chloride transport activity of F508del-CFTR protein." @default.
• W1971859563 created "2016-06-24" @default.
• W1971859563 creator A5016553464 @default.
• W1971859563 creator A5024852710 @default.
• W1971859563 creator A5028875149 @default.
• W1971859563 creator A5050582008 @default.
• W1971859563 creator A5052613378 @default.
• W1971859563 creator A5054386753 @default.
• W1971859563 creator A5059608367 @default.
• W1971859563 date "2008-03-01" @default.
• W1971859563 modified "2023-10-03" @default.
• W1971859563 title "Preclinical Evidence that Sildenafil and Vardenafil Activate Chloride Transport in Cystic Fibrosis" @default.
• W1971859563 cites W120703973 @default.
• W1971859563 cites W1482359363 @default.
• W1971859563 cites W1503660683 @default.
• W1971859563 cites W1524509448 @default.
• W1971859563 cites W1906410030 @default.
• W1971859563 cites W1971650059 @default.
• W1971859563 cites W1971898035 @default.
• W1971859563 cites W1974220359 @default.
• W1971859563 cites W1980024311 @default.
• W1971859563 cites W1983837664 @default.
• W1971859563 cites W1983846584 @default.
• W1971859563 cites W2002423049 @default.
• W1971859563 cites W2010211613 @default.
• W1971859563 cites W2013768658 @default.
• W1971859563 cites W2024183228 @default.
• W1971859563 cites W2025137736 @default.
• W1971859563 cites W2026226671 @default.
• W1971859563 cites W2031854098 @default.
• W1971859563 cites W2032778102 @default.
• W1971859563 cites W2035736227 @default.
• W1971859563 cites W2037341705 @default.
• W1971859563 cites W2039599894 @default.
• W1971859563 cites W2041620145 @default.
• W1971859563 cites W2056041537 @default.
• W1971859563 cites W2061816753 @default.
• W1971859563 cites W2062364311 @default.
• W1971859563 cites W2063427124 @default.
• W1971859563 cites W2076934605 @default.
• W1971859563 cites W2080130466 @default.
• W1971859563 cites W2080423498 @default.
• W1971859563 cites W2109354493 @default.
• W1971859563 cites W2112048001 @default.
• W1971859563 cites W2112515581 @default.
• W1971859563 cites W2115823017 @default.
• W1971859563 cites W2117903697 @default.
• W1971859563 cites W2118462346 @default.
• W1971859563 cites W2119575920 @default.
• W1971859563 cites W2125391335 @default.
• W1971859563 cites W2130371445 @default.
• W1971859563 cites W2132240172 @default.
• W1971859563 cites W2159379871 @default.
• W1971859563 cites W2186537504 @default.
• W1971859563 cites W22857079 @default.
• W1971859563 cites W2316875009 @default.
• W1971859563 cites W4239854002 @default.
• W1971859563 cites W4241884576 @default.
• W1971859563 doi "https://doi.org/10.1164/rccm.200703-344oc" @default.
• W1971859563 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18006891" @default.
• W1971859563 hasPublicationYear "2008" @default.
• W1971859563 type Work @default.
• W1971859563 sameAs 1971859563 @default.
• W1971859563 citedByCount "66" @default.
• W1971859563 countsByYear W19718595632012 @default.
• W1971859563 countsByYear W19718595632013 @default.
• W1971859563 countsByYear W19718595632014 @default.
• W1971859563 countsByYear W19718595632015 @default.
• W1971859563 countsByYear W19718595632016 @default.
• W1971859563 countsByYear W19718595632017 @default.
• W1971859563 countsByYear W19718595632018 @default.
• W1971859563 countsByYear W19718595632019 @default.
• W1971859563 countsByYear W19718595632020 @default.
• W1971859563 countsByYear W19718595632021 @default.
• W1971859563 countsByYear W19718595632022 @default.
• W1971859563 countsByYear W19718595632023 @default.
• W1971859563 crossrefType "journal-article" @default.
• W1971859563 hasAuthorship W1971859563A5016553464 @default.
• W1971859563 hasAuthorship W1971859563A5024852710 @default.
• W1971859563 hasAuthorship W1971859563A5028875149 @default.
• W1971859563 hasAuthorship W1971859563A5050582008 @default.
• W1971859563 hasAuthorship W1971859563A5052613378 @default.
• W1971859563 hasAuthorship W1971859563A5054386753 @default.
• W1971859563 hasAuthorship W1971859563A5059608367 @default.
• W1971859563 hasConcept C126322002 @default.
• W1971859563 hasConcept C134018914 @default.
• W1971859563 hasConcept C145822097 @default.
• W1971859563 hasConcept C150903083 @default.
• W1971859563 hasConcept C178790620 @default.
• W1971859563 hasConcept C185592680 @default.
• W1971859563 hasConcept C207001950 @default.
• W1971859563 hasConcept C24998067 @default.
• W1971859563 hasConcept C2776768464 @default.
• W1971859563 hasConcept C2776938444 @default.
• W1971859563 hasConcept C2778428886 @default.
• W1971859563 hasConcept C2778822586 @default.
• W1971859563 hasConcept C2779276759 @default.
• W1971859563 hasConcept C2779608910 @default.

• next