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- W1971875616 abstract "The RNA segment (Gene 10) from a human group B rotavirus which encodes the homologue of the rotavirus enterotoxin (NSP4) has been cloned and sequenced. The gene is of the same length (751 nucleotides) as its better-characterized group A rotavirus counterpart but shows minimal homology (approximately 10%) to it at the primary sequence level. Despite this low level of sequence homology, secondary structure predictions for the group B protein (ADRV-NSP4) showed a close similarity of structural features with the group A protein. Full-length ADRV-NSP4 was expressed in Escherichia coli with an amino terminal 6xHis tag that was used to purify it to homogeneity. The cytotoxicity of the purified protein was examined in a rapid dye-uptake assay that assesses membrane permeability and was found to be comparable to its group A counterpart." @default.
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- W1971875616 date "2005-06-01" @default.
- W1971875616 modified "2023-09-25" @default.
- W1971875616 title "Molecular characterization of the rotavirus NSP4 enterotoxin homologue from group B rotavirus" @default.
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- W1971875616 doi "https://doi.org/10.1016/j.virusres.2005.02.005" @default.
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