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• W1971937081 abstract "The influence of medium composition and cultural conditions on simultaneous yield of mycelia, intracellular polysaccharide, adenosine, and mannitol by Cordyceps militaris CGMCC 2909 was investigated with desirability functions in this study. An optimization strategy based on the desirability function approach, together with response surface methodology (RSM) has been used to optimize medium composition, and the optimal medium was obtained via the desirability as follows: yeast extract 10.33 g/L, sucrose 27.24 g/L, KH2PO4 5.60 g/L and the optimal culture conditions are initial pH 6, 25°C, rotation speed 150 r/minute, inoculum size 4%(v/v), and medium capacity 40 mL/250 mL. Under these conditions, the yield of mycelia, intracellular polysaccharide, adenosine and mannitol reached 12.19 g/L, 0.6 g/L, 61.84 mg/L, and 1.38 g/L, respectively, and the D value was 0.77. Furthermore, the polysaccharides showed significant antitumor activities against HeLa and HepG2 in vitro in a dose-dependent manner in 72 hours. At a concentration of 1000 mg/mL, the inhibition rate of polysaccharides was 92.38% and 98.79%. The IC50 for HeLa and HepG2 were 70.91 μg/mL and 97.63 μg/mL, respectively." @default.
• W1971937081 created "2016-06-24" @default.
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• W1971937081 date "2014-12-01" @default.
• W1971937081 modified "2023-09-30" @default.
• W1971937081 title "Optimization of fermentation process of Cordyceps militaris and antitumor activities of polysaccharides in vitro" @default.
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• W1971937081 doi "https://doi.org/10.1016/j.jfda.2014.01.028" @default.
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