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- W1971937215 abstract "Locust bean gum and guar gum are galactomannans used as additives (E 410 and E 412, respectively) in the food industry as stabilizing agents. Analytical discrimination between the two additives in gums and foods is now feasible by molecular techniques. However, only complex and time-consuming DNA isolation protocols are available to date. We have developed simple improved protocols to obtain enough DNA suitable for PCR amplification from a few milligrams of commercial E 410 and E 412 additives (containing more than 75% polysaccharides). The suspension of additives in water or 10 mM Tris-HCl, pH 8.5, efficiently recovers DNA suitable for authentication in PCR assays. However, the Tris method was much more efficient for the extraction of DNA from E 410 than for E 412 additives. Conversely, the water method was the most suitable for detecting DNA extracted from E 412 or from E 410/E 412 mixtures. Combined with the use of the two specific ribosomal primer pairs previously designed, our methods are well-suited for a fast and simple high-throughput sample treatment of commercial gums for molecular certification. Keywords: Guar; locust bean; gums; molecular certification" @default.
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- W1971937215 date "2005-03-25" @default.
- W1971937215 modified "2023-10-01" @default.
- W1971937215 title "New Method of DNA Isolation from Two Food Additives Suitable for Authentication in Polymerase Chain Reaction Assays" @default.
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- W1971937215 doi "https://doi.org/10.1021/jf0482919" @default.
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