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- W1971994212 abstract "Practical aspects of the analysis of amino acids are addressed in this paper. Conditions for the resolution of enantionmers of all teh common amino acids as their Dns derivatives using the chiral mobile phase additive: l-prolyl-n-octylamide-Ni(II) are reported. Variable d,l selectivity is obsserved, while maintaining good column efficiency, l-prolyl-n-dodecylamide-Ni(II) is found to distribute on the surface of a n-alkyl-bonded stationary phase and may act as a chiral immobilized phase. The separation of all the common Dns-amino acids within ≈ 35 min using reversed-phase conditions and a linear gradient is presented. Approaches to enantiomeric composition analysis of amino acids in a mixture are discussed, and a coupled column strategy linking the two separations reported above is proposed. In the combined system amino acid analysis is performed in the first part, with switching oof individual amino acids of interest to the second part of the system for d,l-enantiomeric composition determination." @default.
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- W1971994212 date "1981-01-01" @default.
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- W1971994212 title "Practical considerations in the chiral separation of dns-amino acids by reversed-phase liquid chromatography using metal chelated additives" @default.
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- W1971994212 doi "https://doi.org/10.1016/s0021-9673(00)82660-6" @default.
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