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- W1972017079 abstract "Abstract The supercoiled and nicked circular forms of SV40 virus DNA, in a crude preparation from whole infected cells, have been resolved both from one another and from linear DNA by a simple one-stage process. This has been achieved using polyacrylamide gel electrophoresis, a technique hitherto little applied to the analysis of DNA. The separations are assisted by the phenomenon that high molecular weight linear DNA, even of considerable size heterogeneity, is concentrated into a narrow band on gel electrophoresis. A factor which greatly assists resolution of the circular DNA species is the differential effect of varying acrylamide concentration in the gel on the respective electrophoretic mobility of linear as compared with circular forms of DNA. It is proposed that this approach should serve as a simple and versatile means of identifying circular DNA species in simple whole cell DNA extracts." @default.
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- W1972017079 date "1973-04-01" @default.
- W1972017079 modified "2023-09-27" @default.
- W1972017079 title "The identification of the circular forms of SV40 DNA in whole infected cell preparations" @default.
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- W1972017079 doi "https://doi.org/10.1016/0042-6822(73)90334-6" @default.
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