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• W1972117075 startingPage "272" @default.
• W1972117075 abstract "A Bacillus sp., isolated from an exotic soil from the Northwestern Himalayas, when incubated with exogenous progesterone for 120 h, produced two metabolites. The Bacillus sp. was identified as Bacillus sphaericus on the basis of morphology, biochemical characteristics and 16SrDNA sequence. Qualitative and quantitative analyses of progesterone and biotransformation products were carried out by TLC, HPLC, and HPLC-MS. The purified compounds were identified by IR and 1H NMR spectra as 1,4-androstadiene-3,17-dione (ADD) and 4-androstene-3,17-dione (AD). Some 91% of the input progesterone was biotransformed to AD and ADD in nutrient broth (pH 7, 220 rpm) at 28°C with a substrate concentration of 0.5 g L−1. Use of surfactants inhibited the biotransformation process." @default.
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• W1972117075 date "2008-01-01" @default.
• W1972117075 modified "2023-10-10" @default.
• W1972117075 title "Production of 17-keto androstene steroids by the side chain cleavage of progesterone with<i>Bacillus</i><i>sphaericus</i>" @default.
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• W1972117075 doi "https://doi.org/10.1080/10242420701707637" @default.
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