Matches in SemOpenAlex for { <https://semopenalex.org/work/W1972137712> ?p ?o ?g. }
- W1972137712 endingPage "375" @default.
- W1972137712 startingPage "367" @default.
- W1972137712 abstract "Extracellular components secreted by Bifidobacterium breve C50 can induce maturation, high IL-10 production and prolonged survival of dendritic cells via a TLR2 pathway. In this study, the components were isolated from the supernatant by gel filtration chromatography. Antibodies raised against the major compounds with molecular weight above 600 kDa (Bb C50BC) also recognized compounds of lower molecular weight (200–600 kDa). TLR2 and TLR6 bound to the components already recognized by the antibodies. Trypsin digestion of Bb C50BC released three major peptides whose sequences displayed close similarities to a putative secreted protein with a CHAP amidase domain from B. breve. The 1300-bp genomic region corresponding to the hypothetical protein was amplified by PCR. The deduced polypeptide started with an N-terminal signal sequence of 45 amino acids, containing the lipobox motif (LAAC) with the cysteine in position 25, and 2 positively charged residues within the first 14 residues of the signal sequence. Lipid detection in Bb C50BC by GC/MS further supported the implication of a lipoprotein. Sugars were also detected in Bb C50BC. Close similarity with the glucan-binding protein B from Bifidobacterium animalis of two released peptides from Bb C50BC protein suggested that glucose moieties, possibly in glucan form, could be bound to the lipoprotein. Finally, heating at 100 °C for 5 min led to the breakdown of Bb C50BC in compounds of molecular weight below 67 kDa, which suggested that Bb C50BC was an aggregate. One might assume that a basic unit was formed by the lipoprotein bound putatively to glucan. Besides the other sugars and hexosamines recognized by galectin 1 were localized at the surface of the Bb C50BC aggregate. In conclusion, the extracellular components secreted by B. breve C50 were constituted of a lipoprotein putatively associated with glucose moieties and acting in an aggregating form as an agonist of TLR2/TLR6." @default.
- W1972137712 created "2016-06-24" @default.
- W1972137712 creator A5045153620 @default.
- W1972137712 creator A5047634656 @default.
- W1972137712 creator A5051336652 @default.
- W1972137712 creator A5065755262 @default.
- W1972137712 creator A5089215095 @default.
- W1972137712 date "2014-12-01" @default.
- W1972137712 modified "2023-10-18" @default.
- W1972137712 title "Bifidobacterium breve C50 secretes lipoprotein with CHAP domain recognized in aggregated form by TLR2" @default.
- W1972137712 cites W1458394572 @default.
- W1972137712 cites W1484908957 @default.
- W1972137712 cites W1594700207 @default.
- W1972137712 cites W1638424324 @default.
- W1972137712 cites W1733249062 @default.
- W1972137712 cites W1775749144 @default.
- W1972137712 cites W1967612113 @default.
- W1972137712 cites W1968530421 @default.
- W1972137712 cites W1970068894 @default.
- W1972137712 cites W1973702400 @default.
- W1972137712 cites W1975539423 @default.
- W1972137712 cites W1976980195 @default.
- W1972137712 cites W1992173766 @default.
- W1972137712 cites W1993121538 @default.
- W1972137712 cites W1995744610 @default.
- W1972137712 cites W1999164610 @default.
- W1972137712 cites W2021310231 @default.
- W1972137712 cites W2022715414 @default.
- W1972137712 cites W2025980374 @default.
- W1972137712 cites W2033605140 @default.
- W1972137712 cites W2042433863 @default.
- W1972137712 cites W2045141190 @default.
- W1972137712 cites W2046223523 @default.
- W1972137712 cites W2047112721 @default.
- W1972137712 cites W2056010853 @default.
- W1972137712 cites W2056626440 @default.
- W1972137712 cites W2056812430 @default.
- W1972137712 cites W2060259273 @default.
- W1972137712 cites W2067074405 @default.
- W1972137712 cites W2069663822 @default.
- W1972137712 cites W2078314435 @default.
- W1972137712 cites W2084758459 @default.
- W1972137712 cites W2093304828 @default.
- W1972137712 cites W2095936458 @default.
- W1972137712 cites W2110834028 @default.
- W1972137712 cites W2120755207 @default.
- W1972137712 cites W2124334639 @default.
- W1972137712 cites W2125470025 @default.
- W1972137712 cites W2132594700 @default.
- W1972137712 cites W2137015675 @default.
- W1972137712 cites W2138875176 @default.
- W1972137712 cites W2140776090 @default.
- W1972137712 cites W2142855591 @default.
- W1972137712 cites W2146796201 @default.
- W1972137712 cites W2151242602 @default.
- W1972137712 cites W2154202919 @default.
- W1972137712 cites W2169950761 @default.
- W1972137712 cites W2341541815 @default.
- W1972137712 cites W2400378995 @default.
- W1972137712 doi "https://doi.org/10.1016/j.biochi.2014.10.007" @default.
- W1972137712 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25457102" @default.
- W1972137712 hasPublicationYear "2014" @default.
- W1972137712 type Work @default.
- W1972137712 sameAs 1972137712 @default.
- W1972137712 citedByCount "3" @default.
- W1972137712 countsByYear W19721377122016 @default.
- W1972137712 countsByYear W19721377122017 @default.
- W1972137712 countsByYear W19721377122018 @default.
- W1972137712 crossrefType "journal-article" @default.
- W1972137712 hasAuthorship W1972137712A5045153620 @default.
- W1972137712 hasAuthorship W1972137712A5047634656 @default.
- W1972137712 hasAuthorship W1972137712A5051336652 @default.
- W1972137712 hasAuthorship W1972137712A5065755262 @default.
- W1972137712 hasAuthorship W1972137712A5089215095 @default.
- W1972137712 hasConcept C100544194 @default.
- W1972137712 hasConcept C104317684 @default.
- W1972137712 hasConcept C10858879 @default.
- W1972137712 hasConcept C153911025 @default.
- W1972137712 hasConcept C167625842 @default.
- W1972137712 hasConcept C185592680 @default.
- W1972137712 hasConcept C2778163477 @default.
- W1972137712 hasConcept C2779707754 @default.
- W1972137712 hasConcept C2780072125 @default.
- W1972137712 hasConcept C2780206646 @default.
- W1972137712 hasConcept C2780305828 @default.
- W1972137712 hasConcept C55493867 @default.
- W1972137712 hasConcept C86803240 @default.
- W1972137712 hasConceptScore W1972137712C100544194 @default.
- W1972137712 hasConceptScore W1972137712C104317684 @default.
- W1972137712 hasConceptScore W1972137712C10858879 @default.
- W1972137712 hasConceptScore W1972137712C153911025 @default.
- W1972137712 hasConceptScore W1972137712C167625842 @default.
- W1972137712 hasConceptScore W1972137712C185592680 @default.
- W1972137712 hasConceptScore W1972137712C2778163477 @default.
- W1972137712 hasConceptScore W1972137712C2779707754 @default.
- W1972137712 hasConceptScore W1972137712C2780072125 @default.
- W1972137712 hasConceptScore W1972137712C2780206646 @default.
- W1972137712 hasConceptScore W1972137712C2780305828 @default.