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• W1972141494 abstract "Summary To investigate the usefulness of follicular fluid ( FF ) in relation to blood plasma and bile as indicators of exposure of dairy cows to ZEN , DON and their metabolites, a dose–response study was performed with 30 dairy cows. The cows, 10 in each group (named CON ; FUS ‐50, FUS ‐100), received a diet with three different concentrations of Fusarium toxin‐contaminated maize. Thereby, the following dietary concentration were reached: CON (0.02 mg ZEN and 0.07 mg DON , per kg dry matter, DM ), FUS ‐50 (0.33 mg ZEN and 2.62 mg DON , per kg DM ) and FUS ‐100 (0.66 mg ZEN and 5.24 mg DON , per kg DM ). ZEN , DON and de‐epoxy‐ DON (de‐ DON ) were detected in FF . Based on the linear regression between toxin concentration in plasma and FF , it seems that about 50 % (m = 0.5) of ZEN present in plasma is present in FF while an increase of 1 ng/ml DON or de‐ DON in plasma is paralleled by an increase of 1.5 ng/ml DON or 1.1 ng/ml de‐ DON in FF . ZEN , DON and their metabolites, except zearalenone ( ZAN ), were also detected in bile. Contrary to DON and de‐ DON , ZEN and its metabolites were accumulated in bile so that the concentration of ZEN and metabolites was much higher than for DON and de‐ DON . The main compound was β ‐zearalenol ( β ‐ ZEL ). The biliary ZEN , α ‐zearalenol ( α ‐ ZEL ) and β ‐ ZEL concentration correlated linearly with each other with an uncertainty of <15 % ( r 2 ≥ 0.86), whereas the ratio between ZEN : α ‐ ZEL : β ‐ ZEL was about 1.5:1:11. With the help of established linear relationship between toxin intake and toxin concentration, bile could be used as diagnostic indicator to assess the exposure of cows." @default.
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• W1972141494 date "2014-12-31" @default.
• W1972141494 modified "2023-10-16" @default.
• W1972141494 title "Diagnostic opportunities for evaluation of the exposure of dairy cows to the mycotoxins deoxynivalenol (DON) and zearalenone (ZEN): reliability of blood plasma, bile and follicular fluid as indicators" @default.
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• W1972141494 doi "https://doi.org/10.1111/jpn.12285" @default.
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