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- W1972151452 abstract "Transport of BPV-1 virus from the cell membrane to the nucleus was studied in vitro in CV-1 cells. At reduced temperature (4 degrees C), BPV-1 binding to CV-1 cells was unaffected but there was no transport of virions across the cytosol. Electron microscopy showed BPV-1 virions in association with microtubules in the cytoplasm, a finding confirmed by co-immunoprecipitation of L1 protein and tubulin. Internalization of virus was unimpaired in cells treated with the microtubule-depolymerizing drug nocodazole but virions were retained in cytoplasmic vesicles and not transported to the nucleus. We conclude that a microtubule transport mechanism in CV-1 cells moves intact BPV-1 virions from the cell surface to the nuclear membrane." @default.
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- W1972151452 date "2001-04-01" @default.
- W1972151452 modified "2023-09-29" @default.
- W1972151452 title "Association of Bovine Papillomavirus Type 1 with Microtubules" @default.
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- W1972151452 doi "https://doi.org/10.1006/viro.2000.0728" @default.
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